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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HDAC3 Antibody (C‑Terminus) LS‑C48559
Antibody
HDAC3 antibody LS-C48559 is an unconjugated rabbit polyclonal antibody to HDAC3 from human, mouse, rat and other species. Validated for IHC and WB.
Catalog
Size
Price
LS-C48559-30
30 µg
Unavailable
LS-C48559-100
100 µg (0.2 mg/ml)
Unavailable

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Immunohistochemical analysis of Histone Deacetylase 3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Immunohistochemical analysis of Histone Deacetylase 3 (pS424) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
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Product Description

HDAC3 antibody LS-C48559 is an unconjugated rabbit polyclonal antibody to HDAC3 from human, mouse, rat and other species. Validated for IHC and WB.
About HDAC3
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4), and some other non-histone substrates. Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. O15379 NM_003883 NP_003874.2

HDAC3 Antibody, RPD3 Antibody, SMAP45 Antibody, HD3 Antibody, Histone deacetylase 3 Antibody, RPD3-2 Antibody


Specifications

Target
Human HDAC3
Host
Rabbit
Reactivity
Human, Mouse, Rat, Chicken (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (20 - 30 µg/ml)
  • Western blot (0.5 - 4 µg/ml)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HDAC3 antibody was raised against synthetic peptide corresponding to the C-terminus of human HDAC-3.
Epitope
C-Terminus
Specificity
The antibody detects the 49 kD HDAC3. It does not cross-react with other HDAC proteins including HDAC1, 2, 4, 5, 6, 7, and 8. Jurkat cell lysate can be used as a positive control. Blocking peptide is also available.
Presentation
PBS, pH 7.2, 30% glycerol, 0.5% BSA, 0.01% Thimerosal.
Storage
Short term: -20°C; Long term: -70°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/20/2018

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