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Antibodies > HDAC3 > LS-C312753
 
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Catalog Number Size Price
LS-C312753-10 10 µg $318 
LS-C312753-100 100 µg (0.5 mg/ml) $470 
HDAC3 Antibody - HDAC3 antibody. IHC(P): Mouse Brain Tissue.
HDAC3 Antibody - HDAC3 antibody. ICC: A549 Cell.
HDAC3 Antibody - HDAC3 antibody. IHC(F): Mouse Brain Tissue.
HDAC3 Antibody - HDAC3 antibody. IHC(P): Human Intestinal Cancer Tissue.
HDAC3 Antibody - HDAC3 antibody. IHC(P): Human Lung Cancer Tissue.
HDAC3 Antibody - IF analysis of HDAC3 using anti-HDAC3 antibody HDAC3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HDAC3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HDAC3 Antibody - IF analysis of HDAC3 using anti-HDAC3 antibody HDAC3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HDAC3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HDAC3 Antibody - Western blot analysis of HDAC3 using anti-HDAC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Stomach Tissue Lysate Lane 2: Rat Testis Tissue Lysate Lane 3: MCF-7 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: JURKAT Cell Lysate Lane 6: SKOV Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
HDAC3 Antibody - WB of HDAC3 antibody. Lane 1: Rat Stomach Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: JURKAT Cell Lysate. Lane 6: SKOV Cell Lysate.
HDAC3 Antibody - Flow Cytometry analysis of A431 cells using anti-HDAC3 antibody. Overlay histogram showing A431 cells stained with anti-HDAC3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HDAC3 Antibody - Flow Cytometry analysis of U937cells using anti-HDAC3 antibody. Overlay histogram showing U937 cells stained with anti-HDAC3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HDAC3 Antibody - HDAC3 antibody. IHC(P): Mouse Brain Tissue.
HDAC3 Antibody - HDAC3 antibody. ICC: A549 Cell.
HDAC3 Antibody - HDAC3 antibody. IHC(F): Mouse Brain Tissue.
HDAC3 Antibody - HDAC3 antibody. IHC(P): Human Intestinal Cancer Tissue.
HDAC3 Antibody - HDAC3 antibody. IHC(P): Human Lung Cancer Tissue.
HDAC3 Antibody - IF analysis of HDAC3 using anti-HDAC3 antibody HDAC3 was detected in immunocytochemical section of Hela cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HDAC3 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HDAC3 Antibody - IF analysis of HDAC3 using anti-HDAC3 antibody HDAC3 was detected in immunocytochemical section of U20S cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-HDAC3 Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
HDAC3 Antibody - Western blot analysis of HDAC3 using anti-HDAC3 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Stomach Tissue Lysate Lane 2: Rat Testis Tissue Lysate Lane 3: MCF-7 Cell Lysate Lane 4: HELA Cell Lysate Lane 5: JURKAT Cell Lysate Lane 6: SKOV Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HDAC3 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
HDAC3 Antibody - WB of HDAC3 antibody. Lane 1: Rat Stomach Tissue Lysate. Lane 2: Rat Testis Tissue Lysate. Lane 3: MCF-7 Cell Lysate. Lane 4: HELA Cell Lysate. Lane 5: JURKAT Cell Lysate. Lane 6: SKOV Cell Lysate.
HDAC3 Antibody - Flow Cytometry analysis of A431 cells using anti-HDAC3 antibody. Overlay histogram showing A431 cells stained with anti-HDAC3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
HDAC3 Antibody - Flow Cytometry analysis of U937cells using anti-HDAC3 antibody. Overlay histogram showing U937 cells stained with anti-HDAC3 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HDAC3 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human HDAC3 Antibody (aa1‑18, IHC, WB) LS‑C312753

Polyclonal Rabbit anti‑Human HDAC3 Antibody (aa1‑18, IHC, WB) LS‑C312753

Submit Review LSBio Guarantee
Antibody:
HDAC3 Rabbit anti-Human Polyclonal (aa1-18) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312753-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
HDAC3 Rabbit anti-Human Polyclonal (aa1-18) Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus
Format:
Unconjugated, Unmodified

Specifications

Description
HDAC3 antibody LS-C312753 is an unconjugated rabbit polyclonal antibody to HDAC3 (aa1-18) from human. It is reactive with human, mouse, rat and other species. Validated for IHC and WB.
Target
Human HDAC3
Synonyms
HDAC3 | RPD3 | SMAP45 | HD3 | Histone deacetylase 3 | RPD3-2
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bat, Guinea pig, Horse, Pig, Rabbit, Sheep, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human HDAC3(411-428 aa NEFYDGDHDNDKESDVEI), identical to the related rat and mouse sequences.
Epitope
aa1-18
Specificity
Widely expressed.
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HDAC3
O15379 NM_003883 NP_003874.2

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IHC‑plus™ HDAC3 Antibody (aa224‑428, clone 3A7B5) LS‑B3893
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Applications: IHC, IHC - Paraffin, Western blot, ELISA
HDAC3 Antibody - Anti-HDAC3 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:200.
IHC‑plus™ HDAC3 Antibody (clone 7G6C5) LS‑B3894
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, ELISA
HDAC3 Antibody - Immunohistochemical analysis of Histone Deacetylase 3 (pS424) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
HDAC3 Antibody (phospho‑Ser424) LS‑C359014
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
HDAC3 Antibody - Detection of Human HDAC3 by Immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HDAC3 used at a dilution of 1:200 (1 Detection: DAB.
HDAC3 Antibody (aa375‑C‑term) LS‑C287353
Species: Human, Mouse
Applications: IHC, Western blot, Immunoprecipitation
HDAC3 ELISA Kit
Human HDAC3 (Sandwich ELISA) ELISA Kit - LS-F55924
Reactivity: Human
Range: 0.156-10 ng/ml
See all HDAC3 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/25/2024
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