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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HDAC2 Antibody (phospho‑Ser394) LS‑C205264
HDAC2 antibody LS-C205264 is an unconjugated rabbit polyclonal antibody to HDAC2 from human, mouse and rat. Validated for IF, IHC and WB.
Catalog
Size
Price
LS-C205264-50
50 µg (1 mg/ml)
Unavailable

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Product Description

HDAC2 antibody LS-C205264 is an unconjugated rabbit polyclonal antibody to HDAC2 from human, mouse and rat. Validated for IF, IHC and WB.
About HDAC2
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. NM_001527 NP_001518.3

HDAC2 Antibody, HD2 Antibody, Histone deacetylase 2 Antibody, RPD3 Antibody, YAF1 Antibody, YY1-associated factor 1 Antibody


Specifications

Target
Human HDAC2
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HDAC2 antibody was raised against peptide sequence around phosphorylation site of serine 394 (E-D-S (p) -G-D) of human HDAC2.
Epitope
pSer394
Specificity
The antibody detects endogenous levels of HDAC2 only when phosphorylated at serine 394.
Presentation
PBS, pH 7.4, 0.02% sodium azide, 50% glycerol.
Storage
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.
Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.

Western blot

Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.
Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.
Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.

Western blot

Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.
Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.
Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.

Western blot

Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.
Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.

Immunohistochemistry

Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).
Immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC2 (Phospho-Ser394) antibody (left) or the same antibody preincubated with blocking peptide (right).

Immunofluorescence

Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.
Immunofluorescence of methanol-fixed HeLa cells showing nuclear staining using HDAC2 (Phospho-Ser394) antibody.

Western blot

Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.
Western blot of extracts from 293 cells untreated or treated with EGF using HDAC2 (Phospho-Ser394) antibody.


Requested From: 
Date Requested: 6/18/2018

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