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Anti-HDAC2 Antibody (aa410-439) LS-C166821

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HDAC2 Antibodies

Anti-HDAC2 Antibody (aa419-468) IHC-plus™ LS-B965
Rabbit Polyclonal to Human HDAC2
Human, Monkey, Mouse, Bovine, Pig, Rabbit, Chicken
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-HDAC2 Antibody (aa464-478, Magnetic beads) LS-C172205
Rabbit Polyclonal to Human HDAC2
Human, Mouse, Rat
Western blot, Immunoprecipitation
Magnetic beads Conjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HDAC2
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated

Details

Human HDAC2
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Immunofluorescence (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

Specificity and Use

HDAC2 antibody was raised against kLH-conjugated synthetic peptide between amino acids 410-439 from internal region of human HDAC2.
LS-E10303 - Lyophilized - 100 µg - $145.00
aa410-439
Human HDAC2

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About HDAC2

NM_001527

HDAC2 Antibody, HD2 Antibody, Histone deacetylase 2 Antibody, RPD3 Antibody, YAF1 Antibody, YY1-associated factor 1 Antibody

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR.

Immunohistochemistry

Immunohistochemistry
HDAC2 Antibody immunohistochemistry of formalin-fixed and paraffin-embedded human lung carcinoma followed by peroxidase-conjugated secondary antibody and DAB staining.

Immunofluorescence

Immunofluorescence
Fluorescent confocal image of HeLa cell stained with HDAC2 Antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with HDAC2 primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). HDAC2 immunoreactivity is localized to Nucleus significantly.

Western blot

Western blot
HDAC2 Antibody western blot of SH-SY5Y cell line lysates (35 ug/lane). The HDAC2 antibody detected the HDAC2 protein (arrow).

Flow Cytometry

Flow Cytometry
HDAC2 Antibody flow cytometry of K562 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 12/6/2016

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