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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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HDAC10 Antibody (clone 5E2) LS‑C114863
HDAC10 antibody LS-C114863 is an unconjugated mouse monoclonal antibody to human HDAC10. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C114863-100
100 µl
Unavailable

Popular HDAC10 Products

Antibody
Species: Human, Mouse, Rat
Applications: Western blot
IHC of paraffin-embedded Carcinoma of prostate tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot
Immunohistochemical staining of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
IHC of paraffin-embedded Carcinoma of bladder tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
Immunohistochemical staining of paraffin-embedded Ovary tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry

Product Description

HDAC10 antibody LS-C114863 is an unconjugated mouse monoclonal antibody to human HDAC10. Validated for Flow, IHC and WB.
About HDAC10
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Q969S8 NM_032019 NP_114408.3

HDAC10 Antibody, DKFZP761B039 Antibody, HD10 Antibody, Histone deacetylase 10 Antibody


Specifications

Target
Human HDAC10
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal [5E2]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50)
  • Western blot (1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
HDAC10 antibody was raised against full length human recombinant protein of human HDAC10 (NP_114408) produced in HEK293T cell.
Specificity
Human HDAC10
Usage
Concentration: 0.5-1.0 mg/ml (Lot dependent)
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Immunohistochemistry

IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded liver tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Immunohistochemistry

IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).
IHC of paraffin-embedded pancreas tissue using anti-HDAC10 mouse monoclonal antibody. (Dilution 1:50).

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC10 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC10.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-HDAC10 antibody, (Red) compared to a nonspecific negative control antibody (Blue).


Requested From: 
Date Requested: 6/20/2018

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