HDAC1 antibody LS-C7347 is an unconjugated rabbit polyclonal antibody to HDAC1 from human, mouse and rat. Validated for ICC, IP and WB.
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein A purified
- Western blot
- (applications tested for the base form of this product only)
HDAC1 antibody was raised against 6X-His-tagged fragment of mouse histone deacetylase 1 (HDAC 1) representing amino acids 53-482.
Recognizes and is specific for HDAC 1 of a ~65kD. of mouse, rat and human.
Western Blot Analysis: 0.5 ug/ml lot detects HDAC 1in RIPA lysates from 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC 1 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: 3T3 RIPA lysate. Use 20 ug per lane for minigels. Immunocytochemistry: This antibody has been reported to immunostain HDAC 1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells. Immunoprecipitation: 5 ug immunoprecipitates HDAC 1 from 500 ug of 3T3 RIPA lysate. This antibody has been reported to Immunoprecipitate active HDAC.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
For research use only.