Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
(applications tested for the base form of this product only)
HDAC1 antibody was raised against 6X-His-tagged fragment of mouse histone deacetylase 1 (HDAC 1) representing amino acids 53-482.
Recognizes and is specific for HDAC 1 of a ~65kD. of mouse, rat and human.
Western Blot Analysis: 0.5 ug/ml lot detects HDAC 1in RIPA lysates from 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC 1 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: 3T3 RIPA lysate. Use 20 ug per lane for minigels. Immunocytochemistry: This antibody has been reported to immunostain HDAC 1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells. Immunoprecipitation: 5 ug immunoprecipitates HDAC 1 from 500 ug of 3T3 RIPA lysate. This antibody has been reported to Immunoprecipitate active HDAC.
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function.