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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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HDAC1 Antibody (aa53‑482) LS‑C7347
HDAC1 antibody LS-C7347 is an unconjugated rabbit polyclonal antibody to HDAC1 from human, mouse and rat. Validated for ICC, IP and WB.
Antibody
Catalog
Size
Price
LS-C7347-200
200 µg (1 mg/ml)
$725

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Product Description

HDAC1 antibody LS-C7347 is an unconjugated rabbit polyclonal antibody to HDAC1 from human, mouse and rat. Validated for ICC, IP and WB.

Specifications

Target
Mouse HDAC1
Synonyms
HDAC1, Histone deacetylase 1, GON-10, HD1, RPD3, RPD3L1
Host
Rabbit
Reactivity
Mouse, Human, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • ICC
  • Western blot
  • Immunoprecipitation
  • (applications tested for the base form of this product only)
Immunogen
HDAC1 antibody was raised against 6X-His-tagged fragment of mouse histone deacetylase 1 (HDAC 1) representing amino acids 53-482.
Epitope
aa53-482
Specificity
Recognizes and is specific for HDAC 1 of a ~65kD. of mouse, rat and human.
Usage
Western Blot Analysis: 0.5 ug/ml lot detects HDAC 1in RIPA lysates from 3T3 cells. 3T3 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-HDAC 1 (0.5 ug/ml). Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Positive Antigen Control: 3T3 RIPA lysate. Use 20 ug per lane for minigels. Immunocytochemistry: This antibody has been reported to immunostain HDAC 1 in 1% paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 3T3 cells. Immunoprecipitation: 5 ug immunoprecipitates HDAC 1 from 500 ug of 3T3 RIPA lysate. This antibody has been reported to Immunoprecipitate active HDAC.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About HDAC1
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Q13547 NM_004964 NP_004955.2


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Requested From: United States
Date Requested: 10/15/2018

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