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Anti-HDAC1 Antibody (aa467-482) IHC-plus™ LS-B3438

Note: This antibody replaces LS-C7350

Ordering

Wt. Vol. Conc. Price
50 µg - 0.25 mg/ml $395

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

HDAC1 Antibody LS-B3438 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-HDAC1 antibody IHC of human colon.
Immunohistochemistry
Anti-HDAC1 antibody IHC of human breast.

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Mouse Monoclonal (IgG1) to Human HDAC1
Human, Monkey, Mouse, Hamster, Rabbit
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated

Details

Human HDAC1
Mouse
Human, Monkey, Mouse, Hamster, Rabbit (tested or 100% immunogen sequence identity)
Rat, Bovine, Horse, Pig (at least 90% immunogen sequence identity)
IgG1 Monoclonal
Protein G purified

Applications

IHC - Paraffin (5 µg/ml), ICC, Western blot, Immunoprecipitation

Specificity and Use

HDAC1 antibody was raised against a synthetic peptide, aa467-482 [C-EEKPEAKGVKEEVKLA] to human HDAC1, KLH coupled. The immunizing sequence is identical in mouse. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Hamster, Elephant, Rabbit (100%); Rat, Bovine, Horse, Pig (94%).
aa467-482
Recognizes human HDAC1, Mr 60kD. Species cross-reactivity: mouse.
Immunohistochemistry: LS-B3438 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3438 was determined to be 5 ug/ml.

Packaging

PBS, 0.02% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About HDAC1

Q13547 NM_004964 NP_004955.2

HDAC1 Antibody, Histone deacetylase 1 Antibody, GON-10 Antibody, HD1 Antibody, RPD3 Antibody, RPD3L1 Antibody


Immunohistochemistry

Immunohistochemistry
Anti-HDAC1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3438 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-HDAC1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3438 concentration 5 ug/ml.

Requested From: United States
Date Requested: 7/5/2015

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