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HBP1 Antibody (aa396‑445) LS‑C109686
HBP1 antibody LS-C109686 is an unconjugated rabbit polyclonal antibody to HBP1 from human, rat, dog and other species. Validated for WB.
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Species:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications:
IHC, IHC - Paraffin, Western blot
Species:
Human, Monkey, Mouse, Rat
Applications:
IHC, Western blot, ELISA
Product Description
HBP1 antibody LS-C109686 is an unconjugated rabbit polyclonal antibody to HBP1 from human, rat, dog and other species. Validated for WB.
About HBP1
Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
O60381
NM_012257
NP_036389.2
HBP1 Antibody, HMG box-containing protein 1 Antibody, HMG box transcription factor 1 Antibody, HMG-box transcription factor 1 Antibody, USMCC2 Antibody
Specifications
Target
Human HBP1
Host
Rabbit
Reactivity
Human, Monkey, Rat, Dog, Guinea pig
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Bat, Bovine, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
- Western blot (0.2 - 1 µg/ml)
- (applications tested for the base form of this product only)
Immunogen
HBP1 antibody was raised against synthetic peptide located between aa396-445 of human HBP1 (O60381, NP_036389). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog (100%); Orangutan, Mouse, Bovine, Bat, Rabbit, Horse, Pig (92%); Rat, Opossum (85%); Guinea pig (84%).
Blocking Peptide
LS-E11427 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109686. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Immunizing peptide used to generate LS-C109686. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa396-445
Specificity
Human HBP1
Usage
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Images
Western blot
HBP1 antibody LS-C109686 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
HBP1 antibody LS-C109686 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
HBP1 antibody LS-C109686 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Western blot
HBP1 antibody LS-C109686 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Requested From:
Date Requested: 6/19/2018
Date Requested: 6/19/2018
