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Anti-HBP1 Antibody (aa396-445) LS-C109686

Catalog	Size	Price
LS-C109686-50	50 µg	Unavailable

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Western blot of Phospho-HBP1 (S402) antibody

Anti-HBP1 Antibody (aa400-480) LS-C383885

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Anti-HBP1 Antibody (aa340-420) LS-C383886

Antibody:	Rabbit Polyclonal (IgG) to Human HBP1
Reactivity:	Human, Monkey
Application:	IHC, Western blot, ELISA
Format:	Unconjugated

Antibody:	Rabbit Polyclonal to Human HBP1
Reactivity:	Human, Monkey, Rat, Dog, Guinea pig
Application:	Western blot (applications tested for the base form of this product only)
Format:	Unconjugated

HBP1 antibody LS-C109686 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Details

Target:	Human HBP1
Host:	Rabbit
Reactivity:	Human, Monkey, Rat, Dog, Guinea pig (tested or 100% immunogen sequence identity)
Predicted:	Mouse, Bat, Bovine, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality:	Polyclonal
Conjugations:	Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification:	Immunoaffinity purified
Modifications:	Unmodified

Applications

Approved:

Western blot (0.2 - 1 µg/ml)
(applications tested for the base form of this product only)

Specificity and Use

Immunogen:

HBP1 antibody was raised against synthetic peptide located between aa396-445 of human HBP1 (O60381, NP_036389). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Elephant, Dog (100%); Orangutan, Mouse, Bovine, Bat, Rabbit, Horse, Pig (92%); Rat, Opossum (85%); Guinea pig (84%).

Blocking Peptide:

LS-E11427 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C109686. Useful for pre-absorption and neutralization of the antibody's antigen binding site.

Epitope:

aa396-445

Specificity:

Human HBP1

Usage:

Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.

Packaging

Presentation:	Lyophilized from PBS with 2% sucrose
Reconstitution:	Distilled water
Storage:	Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
Restrictions:	For research use only.

About HBP1

Uniprot: O60381 NCBI: NM_012257 NP_036389.2

Synonyms: HBP1 Antibody, HMG box-containing protein 1 Antibody, HMG box transcription factor 1 Antibody, HMG-box transcription factor 1 Antibody, USMCC2 Antibody

Description: Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.

(More About HBP1)

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Western blot

HBP1 antibody LS-C109686 Western blot of Jurkat lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Requested From:
Date Requested: 4/26/2018

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