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Catalog Number Size Price
LS-C153858-100 100 µg (1.22 mg/ml) $502 
HAUS8 Antibody - Western blot of Rabbit Anti-Hice1 pS70 antibody. Lane 1: HeLa cell extracts of untransfected cells (-). Lane 2: transfected HeLa cell extracts with Flag X3-Hice1 WT (WT). Lane 3: transfected HeLa cell extracts with Flag X3-Hice1 S70A mutant (70A). Load: 35 ug per lane. Primary antibody: Hice1 pS70 antibody at 0.5 g/mL for overnight at 4C. Secondary antibody: IRDye800 Conjugated Goat Anti-Rabbit IgG secondary antibody at 1:10000 for 45 min at RT. Block: 5% BLOTTO overnight at 4C. Predicted/Observed size: 44.8 kDa, 48 kDa for Hice1 pS70. Other band(s): none.

Polyclonal Rabbit anti‑Human HAUS8 Antibody (phospho‑Ser70, WB) LS‑C153858

Polyclonal Rabbit anti‑Human HAUS8 Antibody (phospho‑Ser70, WB) LS‑C153858

Antibody:
HAUS8 Rabbit anti-Human Polyclonal (pSer70) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$502
LS-C153858-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
HAUS8 Rabbit anti-Human Polyclonal (pSer70) Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
HAUS8 antibody LS-C153858 is an unconjugated rabbit polyclonal antibody to human HAUS8 (pSer70). Validated for ELISA and WB.
Target
Human HAUS8
Synonyms
HAUS8 | DGT4 | NY-SAR-48 | Sarcoma antigen NY-SAR-48 | HICE1
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-Hice1 pS70 Antibody was prepared by repeated immunizations with a phosphorylated synthetic peptide corresponding to the region of amino acids containing serine 70 of Hice1.
Epitope
pSer70
Specificity
The antibody is specific for the phosphorylated form of Hice1. Reactivity with non-phosphorylated human Hice1 is minimal by ELISA and western blot. A BLAST analysis was used to suggest cross reactivity with Hice1 from human based on 100% sequence homology with the immunogen. Reactivity against homologues from other sources is not known.
Applications
  • Western blot (1.22 µg/ml)
  • ELISA (1:17000 - 1:45000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 44.9 kD in size corresponding to human phosphorylated Hice1 protein by western blotting in the appropriate stimulated tissue or cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About HAUS8
Q9BT25 Q9BT25

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/19/2024