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Anti-HAUS8 Antibody LS-C153765

Ordering

Wt. Vol. Conc. Price
- 100 µl - $405
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular HAUS8 Antibodies

Anti-HAUS8 Antibody (phospho-Ser70) LS-C153858
Rabbit Polyclonal (IgG) to Human HAUS8
Human
Western blot, ELISA
Unconjugated
Western blot Image
Anti-HAUS8 Antibody (aa166-360) LS-C185381
Rabbit Polyclonal (IgG) to Human HAUS8
Human
IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-HAUS8 Antibody IHC-plus™ LS-B10617
Rabbit Polyclonal (IgG) to Human HAUS8
Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human HAUS8
Human
Western blot, ELISA
Unconjugated

Details

Human HAUS8
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (1:10000)
  • ELISA

Specificity and Use

HAUS8 antibody was raised against anti-HICE1 was prepared from whole rabbit serum produced by repeated immunizations with a recombinant full length Hice1 protein.
This antibody reacts with endogenous Hice1 protein. A BLAST analysis was used to suggest reactivity with Hice1 from human based on a 100% homology with the immunizing sequence. Expect reactivity with Hice1 from chimpanzee, Sumatran orangutan based on a 90% homology with the immunizing sequence. Cross-reactivity with Hice1 from other sources has not been determined.
This antiserum has been tested for use in ELISA and western blotting using a full length recombinant Hice1 protein. Specific conditions for reactivity and detection of Hice1 should be optimized by the end user. Expect a band approximately ~45 kD in size corresponding to Hice1 by Western Blot in the appropriate cell lysate or extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About HAUS8

Q9BT25 Q9BT25

HAUS8 Antibody, DGT4 Antibody, NY-SAR-48 Antibody, Sarcoma antigen NY-SAR-48 Antibody, HICE1 Antibody

HAUS8 is 1 of 8 subunits of the 390-kD human augmin complex, or HAUS complex. The augmin complex was first identified in Drosophila, and its name comes from the Latin verb 'augmentare,' meaning 'to increase.' The augmin complex is a microtubule-binding complex involved in microtubule generation within the mitotic spindle and is vital to mitotic spindle assembly (Goshima et al., 2008 [PubMed 18443220]; Uehara et al., 2009 [PubMed 19369198]).

Western blot

Western blot
Anti-HICE1 in Western blot of Immunochemicals' Anti-HICE1 Antibody shows detection of a 45 kDa band corresponding to endogenous HICE1 in lysates of S phase HeLa cells silenced for either control Luciferase or HICE1. In right lane (HICE1i): lysates from sh-HICE1 RNAi-treated lentivirus-infected cells. In left lane (GLi): lysates from sh-Luciferase lentivirus-infected cells as control. Anti-HICE1 Antibody was used at 1:10000. Molecular weight estimation was made by comparison by prestained MW markers. ECL was used for detection. Personal communication, Kyung S. Lee, NCI, Bethesda, MD.
 

Requested From: United States
Date Requested: 12/6/2016

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