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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Hamartin / TSC1 Antibody IHC‑plus™ LS‑B7265
Note: This antibody replaces LS-C15640
TSC1 antibody LS-B7265 is an unconjugated mouse monoclonal antibody to human TSC1 (Hamartin). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B7265-50
50 µg (0.5 mg/ml)
Unavailable

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Product Description

TSC1 antibody LS-B7265 is an unconjugated mouse monoclonal antibody to human TSC1 (Hamartin). Validated for ELISA, IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
About Hamartin / TSC1
Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. Q92574 NM_000368 NP_000359.1

TSC1 Antibody, LAM Antibody, Tuberous sclerosis 1 protein Antibody, Tuberous sclerosis 1 Antibody, Hamartin Antibody, KIAA0243 Antibody, TSC Antibody, Tumor suppressor Antibody


Specifications

Target
Human Hamartin / TSC1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Protein A purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Immunoprecipitation (5 - 10 µg/ml)
  • ELISA (0.1 - 1 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Hamartin / TSC1 antibody was raised against recombinant human Hamartin protein.
Specificity
Specific for the ~130-150kD Hamartin protein and shows no reactivity with the related Tuberin protein. This specificity was confirmed with Western blot testing on both Hamartin- and Tuberin-transfected cell lysates. Reactivity was confirmed with a Hamartin-transfected 293 cell line. Species cross-reactivity: human.
Usage
Suitable for use in ELISA, Western Blot, Immunoprecipitation. ELISA: 0.1-1 ug/ml. Western Blot: 1-3 ug/ml. Immunoprecipitation: 5-10 ug/ml.
Presentation
PBS, pH 7.2, 0.1% sodium azide.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Immunohistochemistry

Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.
Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.

Immunohistochemistry

Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.
Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.

Immunohistochemistry

Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.
Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.

Immunohistochemistry

Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.
Anti-TSC1 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7265 concentration 10 ug/ml.


Requested From: 
Date Requested: 6/18/2018

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