Human, Mouse, Rat (tested or 100% immunogen sequence identity)
IHC - Paraffin (10 µg/ml), Immunofluorescence, Western blot (1:500 - 1:1000)
Specificity and Use
H2AX antibody was raised against synthetic peptide from human H2AFX / H2AX.
Amino acids surrounding Ser 139 of human H2AFX
Immunohistochemistry: LS-B1474 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1474 was determined to be 10 ug/ml.