Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal (IgG2a) to Human GZMB / Granzyme B
IHC - Paraffin
Human GZMB / Granzyme B
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin
Specificity and Use
GZMB / Granzyme B antibody was raised against recombinant protein corresponding to the N-terminus of the mature granzyme B.
Recognizes human Granzyme B.
Suitable for use in Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): 1:25-1:50. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes. Positive control: Hodgkin's lymphoma.
15 mM sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.