Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Mouse Monoclonal [clone 4E6] (IgG2a) to Human GZMB / Granzyme B
IHC - Paraffin, Immunofluorescence, Western blot
Mouse Monoclonal (IgG2a) to Human GZMB / Granzyme B
IHC - Paraffin
Human GZMB / Granzyme B
Human (tested or 100% immunogen sequence identity)
Tissue culture supernatant
IHC - Paraffin
Specificity and Use
Recombinant protein corresponding to the N-terminus of the mature granzyme B.
Recognizes human Granzyme B.
Suitable for use in Immunohistochemistry. Immunohistochemistry (Formalin/paraffin): 1:25-1:50. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes. Positive control: Hodgkin's lymphoma.
15 mM sodium azide.
Long term: Add glycerol (40-50%) -20°C; Short term: +4°C.
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.