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Anti-GZMB / Granzyme B Antibody (clone 351927) LS-C67063


Wt. Vol. Conc. Price
100 µg 100 µl - $530
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular GZMB / Granzyme B Antibodies

Anti-GZMB / Granzyme B Antibody (clone GB11) LS-C44295
Mouse Monoclonal [clone GB11] (IgG1) to Human GZMB / Granzyme B
Human, Monkey, Rhesus monkey
IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Anti-GZMB / Granzyme B Antibody (N-Terminus) IHC-plus™ LS-B2783
Rabbit Polyclonal to Human GZMB / Granzyme B
Human, Mouse, Rat
IHC - Paraffin
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 351927] (IgG2a) to Human GZMB / Granzyme B
IHC, Western blot, Flow Cytometry, ELISA


Human GZMB / Granzyme B
Human (tested or 100% immunogen sequence identity)
IgG2a Monoclonal [351927]
Protein G purified


  • IHC
  • Western blot
  • Flow Cytometry

Specificity and Use

GZMB / Granzyme B antibody was raised against nS0-derived rhGranzyme B.
Recognizes human Granzyme B. Shows no cross-reactivity with rhGranzyme A, -H, rmGranzyme B, -C, -D, or -G.
Western Blot, Flow Cytometry, Immunohistochemistry, Direct ELISA. Western Blot: This antibody can be used at 1-2 ug/mL with the appropriate secondary reagents to detect human Granzyme B. Using a colorimetric detection system, the detection limit for rhGranzyme B is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. Flow Cytometry: This antibody was tested for flow cytometry using NK-92 cells. For intracellular staining to detect human Granzyme B, cells must first be fixed and permeabilized using 4% paraformaldehyde and 0.1% saponin in PBS. Dilute this antibody to 25 ug/mL and add 10 ul of the diluted solution to 1-5 x 10^5 cells in a total reaction volume not exceeding 200ul. Following a 30 minute incubation, cells should be washed with 0.1% saponin prior to addition of a secondary developing reagent. The binding of unlabeled monoclonal antibodies may be visualized by adding 10 ul of a 25 ug/mL solution of a secondary developing reagent such as goat anti-mouse IgG conjugated to a fluorochrome. Cells should be washed for a final time in 0.1% saponin prior to flow cytometric analysis. Immunohistochemistry: This antibody was used at a concentration of 8-25 ug/mL with appropriate secondary reagents to detect human Granzyme B in paraformaldehyde-fixed human PBMC. For chromogenic detection of labeling, the use of Cell and Tissue Staining Kits is recommended. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect human Granzyme B. The detection limit for rhGranzyme B is approximately 5 ng/well.


Lyophilized from PBS, 5% trehalose. Reconstitution: Reconstitute, sterile PBS. If 0.2 ml of PBS is used, the antibody concentration will be 500 ug/ml.
100 µl PBS
For research use only.

About GZMB / Granzyme B

P10144 NM_004131 NP_004122.2

GZMB Antibody, CCPI Antibody, CGL-1 Antibody, Cathepsin G-like 1 Antibody, CSP-B Antibody, CTLA-1 Antibody, CTLA1 Antibody, CGL1 Antibody, CSPB Antibody, Fragmentin 2 Antibody, GRB Antibody, HLP Antibody, Human lymphocyte protein Antibody, Fragmentin-2 Antibody, SECT Antibody, Lymphocyte protease Antibody, T-cell serine protease 1-3E Antibody, C11 Antibody, CTSGL1 Antibody, Cytotoxic serine protease B Antibody, Granzyme B Antibody, Granzyme-2 Antibody

This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.

Requested From: United States
Date Requested: 10/27/2016

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