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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GZMB / Granzyme B Antibody (aa2‑32) LS‑C99306
Granzyme B antibody LS-C99306 is an unconjugated rabbit polyclonal antibody to Granzyme B (GZMB) from human and mouse. Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C99306-400
400 µl
Unavailable

Popular GZMB / Granzyme B Products

Antibody
Species: Human, Monkey, Rhesus monkey
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Antibody
Species: Human, Monkey, Rhesus monkey
Applications: IHC, IHC - Frozen, Immunoprecipitation, Flow Cytometry, ELISA
Anti-GZMB / Granzyme B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2783 concentration 10 ug/ml.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin
Anti-GZMB / Granzyme B antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7602 dilution 1:100.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
IHC of paraffin-embedded Human Kidney tissue using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot

Product Description

Granzyme B antibody LS-C99306 is an unconjugated rabbit polyclonal antibody to Granzyme B (GZMB) from human and mouse. Validated for Flow, IHC and WB.
About GZMB / Granzyme B
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. P10144 NM_004131 NP_004122.2

GZMB Antibody, CCPI Antibody, CGL-1 Antibody, Cathepsin G-like 1 Antibody, CSP-B Antibody, CTLA-1 Antibody, CTLA1 Antibody, CGL1 Antibody, CSPB Antibody, Fragmentin 2 Antibody, GRB Antibody, HLP Antibody, Human lymphocyte protein Antibody, Fragmentin-2 Antibody, SECT Antibody, Lymphocyte protease Antibody, T-cell serine protease 1-3E Antibody, C11 Antibody, CTSGL1 Antibody, Cytotoxic serine protease B Antibody, Granzyme B Antibody, Granzyme-2 Antibody


Specifications

Target
Human GZMB / Granzyme B
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8301 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C99306. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa2-32
Specificity
This GZMB antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 2-32 amino acids from the N-terminal region of human GZMB.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.

Western blot

Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.
Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.

Flow Cytometry

GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.

Western blot

Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.
Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.

Flow Cytometry

GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.

Western blot

Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.
Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.

Flow Cytometry

GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.
GZMB Antibody IHC of formalin-fixed and paraffin-embedded human Lymph tissue followed by peroxidase-conjugated secondary antibody and DAB staining.

Western blot

Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.
Western blot of GZMB (arrow) using rabbit polyclonal GZMB Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GZMB gene.

Western blot

Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.
Western blot of GZMB antibody in mouse stomach lysates (35 ug/lane). GZMB (arrow) was detected using the purified antibody.

Flow Cytometry

GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
GZMB Antibody flow cytometry of HL-60 cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/23/2018

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