Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human GYPA / CD235a / Glycophorin A
Western blot, ELISA
Mouse Monoclonal [clone HIR2 (also GA-R2)] (IgG2b,k) to Human GYPA / CD235a / Glycophorin A
Human GYPA / CD235a / Glycophorin A
Human (tested or 100% immunogen sequence identity)
IgG2b,k Monoclonal [HIR2 (also GA-R2)]
Specificity and Use
The HIR2 (also GA-R2) antibody has been tested by flow cytometric analysis of human peripheral blood leukocytes. Binding of this antibody to red cells at high antibody concentration causes cell agglutination. This can be used at less than or equal to 0.03 ug per test. A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
PBS, pH 7.2, 150 mM sodium chloride, 0.09% sodium azide
GYPA Antibody, CD235a Antibody, CD235a antigen Antibody, Glycophorin A (MN blood group) Antibody, Glycophorin A, GPA Antibody, Glycophorin-A Antibody, GPA Antibody, GPErik Antibody, GPSAT Antibody, Glycophorin MiV Antibody, Glycophorin SAT Antibody, HGpMiX Antibody, HGpMiXI Antibody, HGpMiV Antibody, HGpSta(C) Antibody, Glycophorin MiI Antibody, GpMiIII Antibody, MN Antibody, HGpMiIII Antibody, MN sialoglycoprotein Antibody, Sialoglycoprotein alpha Antibody, Mi.V glycoprotein (24 AA) Antibody, PAS-2 Antibody, Glycophorin A Antibody, Glycophorin Sta type C Antibody, MNS Antibody
Glycophorin A is the major intrinsic membrane protein of the erythrocyte. The N-terminal glycosylated segment, which lies outside the erythrocyte membrane, has MN blood group receptors. Appears to be important for the function of SLC4A1 and is required for high activity of SLC4A1. May be involved in translocation of SLC4A1 to the plasma membrane. Is a receptor for influenza virus.
Staining of normal human whole blood with 0.03 ug of FITC Mouse IgG2b isotype control (blue histogram) or 0.03 ug of FITC anti-human CD235a (HIR2) (purple histogram). Cells in the erythrocyte gate were used for analysis. This image was taken for the unconjugated form of this product. Other forms have not been tested.