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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GUK1 / Guanylate Kinase 1 Antibody (clone 1D7) LS‑C172832
Guanylate Kinase 1 antibody LS-C172832 is an unconjugated mouse monoclonal antibody to human Guanylate Kinase 1 (GUK1). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C172832-100
100 µl (0.33 mg/ml)
Unavailable

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Product Description

Guanylate Kinase 1 antibody LS-C172832 is an unconjugated mouse monoclonal antibody to human Guanylate Kinase 1 (GUK1). Validated for Flow, IHC and WB.
About GUK1 / Guanylate Kinase 1
GUK1 / Guanylate Kinase 1 is an enzyme that catalyzes the transfer of a phosphate group from ATP to guanosine monophosphate (GMP) to form guanosine diphosphate (GDP). The encoded protein is thought to be a good target for cancer chemotherapy. Several transcript variants encoding different isoforms have been found for this gene. Q16774 NM_000858 NP_000849.1

GUK1 Antibody, ATP:GMP phosphotransferase Antibody, GMK Antibody, GMP kinase Antibody, Guanylate kinase 1 Antibody, Guanylate kinase Antibody


Specifications

Target
Human GUK1 / Guanylate Kinase 1
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal [1D7]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:150)
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:100)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Full length human recombinant protein of human GUK1 (NP_000849) produced in HEK293T cell.
Specificity
Human GUK1
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.
IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.
IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.
IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.

Immunohistochemistry

IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.
IHC of paraffin-embedded Human pancreas tissue using anti-GUK1 mouse monoclonal antibody.

Western blot

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GUK1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GUK1.

Western blot

Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.
Western blot of extracts (35 ug) from 9 different cell lines by using anti-GUK1 monoclonal antibody.

Flow Cytometry

Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of Jurkat cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).
Flow cytometry of HeLa cells, using anti-GUK1 antibody (Red), compared to a nonspecific negative control antibody (Blue).

Flow Cytometry

HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GUK1 antibody, and then analyzed by flow cytometry.


Requested From: 
Date Requested: 6/22/2018

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