Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rabbit Polyclonal (IgG) to Human GUCY1A1 / GUCY1A3
Human, Mouse, Rat
Rabbit Polyclonal to Human GUCY1A1 / GUCY1A3
IHC - Paraffin, Western blot
Human GUCY1A1 / GUCY1A3
Human (tested or 100% immunogen sequence identity)
Ammonium sulfate precipitation
IHC - Paraffin (1:10 - 1:50)
Western blot (1:1000)
Specificity and Use
LS-E8710 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C98332. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
This Guanylyl Cyclase alpha 1 (GUCY1A3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 269-300 amino acids from the N-terminal region of human Guanylyl Cyclase alpha 1 (GUCY1A3).
PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Soluble guanylate cyclases are heterodimeric proteins that catalyze the conversion of GTP to 3',5'-cyclic GMP and pyrophosphate. The protein encoded by this gene is an alpha subunit of this complex and it interacts with a beta subunit to form the guanylate cyclase enzyme, which is activated by nitric oxide. Several transcript variants encoding a few different isoforms have been found for this gene.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
Western blot of anti-GUCY1A3 antibody in mouse brain tissue lysate (35 ug/lane). GUCY1A3(arrow) was detected using the purified antibody.
Western blot of GUCY1A3 (arrow) using GUCY1A3 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GUCY1A3 gene (Lane 2) (Origene Technologies).