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Anti-GST / Glutathione S-Transferase Antibody LS-C153770

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GST / Glutathione S-Transferase Antibodies

Anti-GST / Glutathione S-Transferase Antibody LS-C19065
Mouse Monoclonal to Schistosoma japonicum GST / Glutathione S-Transferase
Schistosoma japonicum, Mouse
IHC, Western blot, ELISA
Unconjugated
Western blot Image
Anti-GST / Glutathione S-Transferase Antibody (aa1-425, clone 3G10) IHC-plus™ LS-B1697
Mouse Monoclonal [clone 3G10] (IgG1)
Human, Mouse, S. japonicum
IHC - Paraffin, Immunofluorescence, Western blot, Immunoprecipitation
Unconjugated
Immunohistochemistry Image
Anti-GST / Glutathione S-Transferase Antibody (PE) LS-C285411
Rabbit Polyclonal (IgG) to Schistosoma japonicum GST / Glutathione S-Transferase
Schistosoma japonicum, Human
IHC, Western blot, ELISA
PE Conjugated

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Schistosoma japonicum GST / Glutathione S-Transferase
Schistosoma japonicum
Unconjugated

Details

Schistosoma japonicum GST / Glutathione S-Transferase
Goat
Schistosoma japonicum (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Ion exchange chromatography
Unmodified

Specificity and Use

Glutathione-S-Transferase [Schistosoma japonicum].
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Glutathione-S-Transferase [Schistosoma japonicum]. Cross reactivity against Glutathione-S-Transferase from other sources may occur but has not been specifically determined.
This product has been assayed against 1.0 ug of Glutathione-S-Transferase [Schistosoma japonicum] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Rabbit) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for this product.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide, sterile filtered
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About GST / Glutathione S-Transferase

Glutathione S-transferases (GSTs), previously known as ligandins, comprise a family of eukaryotic and prokaryotic phase II metabolic isozymes best known for their ability to catalyze the conjugation of the reduced form of glutathione (GSH) to xenobiotic substrates for the purpose of detoxification. The GST family consists of three superfamilies: the cytosolic, mitochondrial, and microsomal—also known as MAPEG—proteins.

Western blot

Western blot
Goat anti GST antibody was used to detect GST under reducing and non-reducing conditions. Reduced samples of purified GST contained 4% BME and were boiled for 5 minutes. For blot on the left, samples of ~1 and 0.25 ug of protein per lane were run by SDS-PAGE. Protein was transferred to nitrocellulose and probed with Goat anti GST (1:5K in MB-0070, overnight 4°C). Primary antibody was detected with Dylight 649 conjugated Donkey anti Goat (605-743-125 lot 20834 1:10K 1.5 hr RT in MB-070) and imaged on the BioRad VersaDoc imaging system. Blot on right shows a repeat western blot with the same samples (~1 ug per lane, reduced ® and non-reduced (NR) probed 1:1000 dilution of primary antibody and detection using Dylight 549 conjugated Donkey anti goat (605-742-125 lot 21096 1:10K 1.5 hr RT in MB-070)
 

Requested From: United States
Date Requested: 12/10/2016

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