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Anti-GSK3B / GSK3 Beta Antibody (phospho-Ser9) IHC-plus™ LS-B55

Note: This antibody replaces LS-C18919, LS-C66828

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $395

LSBio (Direct) LSBio (Direct)
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GSK3B / GSK3 Beta Antibody LS-B55 Images

[click image for a larger image and more details]
Immunohistochemistry
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary.
Western blot
Anti-GSK3B pS9 Antibody - Western Blot.

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Rabbit Polyclonal to Human GSK3B / GSK3 Beta
Human
IHC - Paraffin, Western blot, Immunoprecipitation, ELISA
Unconjugated

Details

Human GSK3B / GSK3 Beta
Rabbit
Human (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (5 µg/ml), Western blot (1:500 - 1:3000), Immunoprecipitation, ELISA (1:10000 - 1:50000)

Specificity and Use

GSK3B / GSK3 Beta antibody was raised against synthetic peptide from human GSK3B / GSK3 Beta.
pSer9
The aa 4-12 of human GSK3 beta.
Immunohistochemistry: LS-B55 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B55 was determined to be 5 ug/ml.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Long term: -20°C; Short term: +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GSK3B / GSK3 Beta

P49841 NM_002093 NP_002084.2

GSK3B Antibody, GSK-3 beta Antibody, GSK3 beta Antibody, GSK3beta Antibody, GSK3beta isoform Antibody, TPKI Antibody, GSK 3beta Antibody, Tau protein kinase I Antibody, ZGSK-3beta Antibody

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), EIF2B, CTNNB1/beta-catenin, APC, AXIN1, DPYSL2/CRMP2, JUN, NFATC1/NFATC, MAPT/TAU and MACF1. Requires primed phosphorylation of the majority of its substrates.

Immunohistochemistry

Immunohistochemistry
Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B55 concentration 5 ug/ml.

Western blot

Western blot
Anti-GSK3B pS9 Antibody - Western Blot. Western blot of Rabbit-anti-GSK3B pS9 antibody at a 1:1000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kD. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20, lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20. Molecular weight markers confirm a MW of ~49 kD. Use a 1:2000 dilution of HRP Goat-a-Rabbit IgG (LS-C60865) for detection.

Requested From: United States
Date Requested: 8/1/2015

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