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Anti-GRK5 Antibody IHC-plus™ LS-B1813

Note: This antibody replaces LS-C47397

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GRK5 Antibodies

Anti-GRK5 Antibody (Internal) IHC-plus™ LS-A3034
Rabbit Polyclonal to Human GRK5
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-GRK5 Antibody (N-Terminus) IHC-plus™ LS-A3470
Rabbit Polyclonal to Human GRK5
Human
IHC - Paraffin
Unconjugated
Immunohistochemistry Image
Anti-GRK5 Antibody (Internal) IHC-plus™ LS-A3030
Rabbit Polyclonal to Human GRK5
Human, Monkey, Mouse, Rat, Bat, Bovine, Horse, Pig, Chicken
IHC - Paraffin
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GRK5
Human, Mouse, Rat, Bovine, Chicken, Pufferfish, Zebrafish
IHC - Paraffin, Western blot
Unconjugated

Details

Human GRK5
Rabbit
Human, Mouse, Rat, Bovine, Chicken, Pufferfish, Zebrafish (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (20 µg/ml)
  • Western blot (0.5 - 4 µg/ml)

Specificity and Use

GRK5 antibody was raised against a portion of amino acid350-400of human GRK5.
A portion of amino acid 350-400 of human GRK5.
Immunohistochemistry: LS-B1813 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1813 was determined to be 20 ug/ml.

Packaging

PBS, 0.2% gelatin, 0.05% sodium azide.
Short term 4°C, long term aliquot and store at -20°C, avoid freeze thaw cycles.
For research use only.

About GRK5

P34947 NM_005308 NP_005299.1

GRK5 Antibody, FP2025 Antibody, GPRK5 Antibody

Serine/threonine kinase that phosphorylates preferentially the activated forms of a variety of G-protein-coupled receptors (GPCRs). Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their down-regulation. Phosphorylates a variety of GPCRs, including adrenergic receptors, muscarinic acetylcholine receptors (more specifically Gi-coupled M2/M4 subtypes), dopamine receptors and opioid receptors.

Immunohistochemistry

Immunohistochemistry
Anti-GRK5 antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B1813 concentration 20 ug/ml.

Western blot

Western blot
Western blot of human GRK5 in human liver lysate in the 1) absence, 2) presence of immunizing peptide, 3) mouse and 4) rat liver using antibody at 0.5 ug/ml (human), 4.0 ug/ml (mouse) and 2 ug/ml (rat).

Requested From: United States
Date Requested: 12/2/2016

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