(tested or 100% immunogen sequence identity)
GRIA4 / GLUR4 antibody was raised against carboxy terminus peptide of rat GluR4 conjugated to BSA with glutaraldehyde (RQSSGLAVIASDLP). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bat, Bovine, Horse, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus, Trachemys (100%).
Western blot of rat brain shows a single band co-migrating with GluR4 expressed in transfected cells. No cross-reaction with GluR1-3. The antibody appears to recognize GluR4 in several other species, but has not been extensively characterized in these cases. Species Reactivity: rat and mouse.
Can be used for immunocytochemistry using paraformaldehyde or paraformaldehyde/glutaraldehyde fixed tissue with light and electron microscopy. Cryostat and vibratome sections can be used with or without Triton X-100 treatment. Final concentrations used were 1-3 ug/ml. Western blot analysis can be done at final concentrations of 0.5-2.5 ug/ml.
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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