Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
TLE4, BCE1, BCE-1, E(spI), Enhancer of split groucho 4, ESG, ESG4, GRG4, Protein BCE-1, KIAA1261, R-esp2, B lymphocyte gene 1
Human, Mouse, Rat, Hamster, Rabbit, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Monkey, Bat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)
IHC (1:200 - 1:1000)
Western blot (1:200 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GRG4 / TLE4 antibody was raised against synthetic peptide corresponding to aa217-230 (DYSSESKKQKTEEK) of human Groucho 4 (BSA). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Rabbit, Chicken, Lizard, Xenopus (100%); Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Platypus (93%).
Recognizes Groucho 4/TLE4/ESG4 transcription factor at 82.9kD. Species cross-reactivity: Human and rodent.
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:200-1:1000. Immunohistochemistry: 1:200-1:1000.
PBS, pH 7.2, 40% Glycerol, No preservatives added
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression.