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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GRG4 / TLE4 Antibody (aa217‑230) LS‑C7311
TLE4 antibody LS-C7311 is an unconjugated rabbit polyclonal antibody to TLE4 (GRG4) from human, mouse, rat and other species. Validated for IHC and WB.
Antibody
Catalog
Size
Price
LS-C7311-50
50 µg (1 mg/ml)
Unavailable

Popular GRG4 / TLE4 Products

Anti-TLE4 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6530 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Dog, Guinea pig, Horse, Pig, Rabbit, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Human, Small Intestine: Formalin-Fixed Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
Human Small Intestine: Formalin-Fixed, Paraffin-Embedded (FFPE)
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Immunohistochemical analysis of TLE4 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
Species: Human, Mouse, Rat, Chicken
Applications: IHC, IHC - Paraffin, Western blot
Western blot of TLE4 antibody
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA

Product Description

TLE4 antibody LS-C7311 is an unconjugated rabbit polyclonal antibody to TLE4 (GRG4) from human, mouse, rat and other species. Validated for IHC and WB.
About GRG4 / TLE4
Transcriptional corepressor that binds to a number of transcription factors. Inhibits the transcriptional activation mediated by PAX5, and by CTNNB1 and TCF family members in Wnt signaling. The effects of full-length TLE family members may be modulated by association with dominant-negative AES. Essential for the transcriptional repressor activity of SIX3 during retina and lens development and for SIX3 transcriptional auto-repression. Q04727 NM_007005 NP_008936.2

TLE4 Antibody, BCE1 Antibody, BCE-1 Antibody, E(spI) Antibody, Enhancer of split groucho 4 Antibody, ESG Antibody, ESG4 Antibody, GRG4 Antibody, Protein BCE-1 Antibody, KIAA1261 Antibody, R-esp2 Antibody, B lymphocyte gene 1 Antibody


Specifications

Target
Human GRG4 / TLE4
Host
Rabbit
Reactivity
Human, Mouse, Rat, Hamster, Rabbit, Chicken, Xenopus (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC (1:200 - 1:1000)
  • Western blot (1:200 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GRG4 / TLE4 antibody was raised against synthetic peptide corresponding to aa217-230 (DYSSESKKQKTEEK) of human Groucho 4 (BSA). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Mouse, Rat, Hamster, Rabbit, Chicken, Lizard, Xenopus (100%); Marmoset, Elephant, Panda, Bovine, Dog, Bat, Horse, Pig, Opossum, Platypus (93%).
Epitope
aa217-230
Specificity
Recognizes Groucho 4/TLE4/ESG4 transcription factor at 82.9kD. Species cross-reactivity: Human and rodent.
Usage
Suitable for use in Western Blot and Immunohistochemistry. Western Blot: 1:200-1:1000. Immunohistochemistry: 1:200-1:1000.
Presentation
PBS, pH 7.2, 40% glycerol. No preservative added.
Storage
Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 7/20/2018

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