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Anti-GREM1 / Gremlin-1 Antibody (aa147-175) LS-C100381

Ordering

Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GREM1 / Gremlin-1 Antibodies

Anti-GREM1 / Gremlin-1 Antibody (aa162-176) IHC-plus™ LS-B810
Rabbit Polyclonal to Human GREM1 / Gremlin-1
Human
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GREM1 / Gremlin-1 Antibody (clone 4C2) LS-C125371
Mouse Monoclonal [clone 4C2] (IgG) to Human GREM1 / Gremlin-1
Human
Western blot, ELISA
Unconjugated
Anti-GREM1 / Gremlin-1 Antibody (aa51-100) LS-C170282
Rabbit Polyclonal to Rat GREM1 / Gremlin-1
Rat, Human, Mouse
Western blot
Unconjugated
Western blot Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GREM1 / Gremlin-1
Human, Mouse
IHC - Paraffin, Western blot
Unconjugated

Details

Human GREM1 / Gremlin-1
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Ammonium sulfate precipitation
Unmodified

Applications

  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)

  • IHC - Paraffin

Specificity and Use

GREM1 / Gremlin-1 antibody was raised against kLH-conjugated synthetic peptide between amino acids 147-175 from C-terminal region of mouse GREMLIN.
LS-E8088 - Lyophilized - 100 µg - $145.00
aa147-175
IHC-P: Heat induced epitope retrieval Citrate buffer pH 6.0 in microwave is recommended.

Packaging

PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About GREM1 / Gremlin-1

O60565 NM_013372 NP_037504.1

GREM1 Antibody, CKTSF1B1 Antibody, DAN domain family member 2 Antibody, DAND2 Antibody, DRM Antibody, GREMLIN Antibody, Gremlin 1-like protein Antibody, Increased in high glucose-2 Antibody, Gremlin-1 Antibody, PIG2 Antibody, Proliferation-inducing gene 2 Antibody, Gremlin 1 Antibody, IHG-2 Antibody

GREM1 / Gremlin-1 is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins.

Immunohistochemistry

Immunohistochemistry
ISH and IMH demonstrating gremlin mRNA and gremlin protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of gremlin mRNA (C), proliferating PECs of glomerular crescents show a strong expression of gremlin mRNA by ISH (A); and gremlin protein expression by IMH (B); immune competent infiltrating interstitial cells are also strongly positive for gremlin staining (E); and CTGF was also expressed in these glomerular crescentic cells (F).

Immunohistochemistry

Immunohistochemistry
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Immunohistochemistry

Immunohistochemistry
Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.

Western blot

Western blot
Western blot of anti-Gremlin antibody in CEM cell line lysates (35 ug/lane). Gremlin (arrow) was detected using the purified antibody.

Western blot

Western blot
Western blot of anti-Gremlin antibody in mouse liver cell lysate. Gremlin (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
 

Requested From: United States
Date Requested: 12/3/2016

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