Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GREM1 Antibody, CKTSF1B1 Antibody, DAN domain family member 2 Antibody, DAND2 Antibody, DRM Antibody, GREMLIN Antibody, Gremlin 1-like protein Antibody, Increased in high glucose-2 Antibody, Gremlin-1 Antibody, PIG2 Antibody, Proliferation-inducing gene 2 Antibody, Gremlin 1 Antibody, IHG-2 Antibody
GREM1 / Gremlin-1 is a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted glycosylated protein encoded by this gene is likely due to its direct binding to BMP proteins.
ISH and IMH demonstrating gremlin mRNA and gremlin protein in crescentic PECs and tubular epithelial cells of patients with pauci-immune crescentic GN. In comparison with normal renal tissue in which there is no expression of gremlin mRNA (C), proliferating PECs of glomerular crescents show a strong expression of gremlin mRNA by ISH (A); and gremlin protein expression by IMH (B); immune competent infiltrating interstitial cells are also strongly positive for gremlin staining (E); and CTGF was also expressed in these glomerular crescentic cells (F).
Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. Data and protocol courtesy of Dr. Maria E. Burgos of Universidad Austral de Chile.
Western blot of anti-Gremlin antibody in CEM cell line lysates (35 ug/lane). Gremlin (arrow) was detected using the purified antibody.
Western blot of anti-Gremlin antibody in mouse liver cell lysate. Gremlin (arrow) was detected using purified antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Requested From: United States
Date Requested: 10/20/2016