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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GRB2 Antibody (aa89‑118) LS‑C100001
GRB2 antibody LS-C100001 is an unconjugated rabbit polyclonal antibody to human GRB2. Validated for IHC and WB.
Catalog
Size
Price
LS-C100001-400
400 µl
Unavailable

Popular GRB2 Products

Antibody
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Anti-GRB2 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3923 concentration 5 ug/ml.
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Product Description

GRB2 antibody LS-C100001 is an unconjugated rabbit polyclonal antibody to human GRB2. Validated for IHC and WB.
About GRB2
Adapter protein that provides a critical link between cell surface growth factor receptors and the Ras signaling pathway.Isoform 2 does not bind to phosphorylated epidermal growth factor receptor (EGFR) but inhibits EGF-induced transactivation of a RAS-responsive element. Isoform 2 acts as a dominant negative protein over GRB2 and by suppressing proliferative signals, may trigger active programmed cell death. P62993 NM_002086 NP_002077.1

GRB2 Antibody, Abundant SRC homology Antibody, Adapter protein GRB2 Antibody, ASH Antibody, EGFRBP-GRB2 Antibody, MST084 Antibody, MSTP084 Antibody, Protein Ash Antibody, SH2/SH3 adapter GRB2 Antibody, Grb3-3 Antibody, HT027 Antibody, NCKAP2 Antibody


Specifications

Target
Human GRB2
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:100)
  • Western blot (1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Blocking Peptide
LS-E8179 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100001. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa89-118
Specificity
This GRB2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 89-118 amino acids from the Central region of human GRB2.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.
Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.

Western blot

Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.
Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.

Western blot

Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.
Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.

Western blot

Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).

Immunohistochemistry

Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.
Western blot of GRB2 Antibody in A2058 cell line lysates (35 ug/lane). GRB2 (arrow) was detected using the purified antibody.

Western blot

Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).
Western blot of GRB2 (arrow) using GRB2 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GRB2 gene (Lane 2) (Origene Technologies).


Requested From: 
Date Requested: 6/20/2018

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