GRB2 antibody LS-C7289 is an unconjugated mouse monoclonal antibody to GRB2 from human, mouse, rat and other species. Validated for IF, IHC and WB.
Human, Mouse, Rat, Bovine, Xenopus
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence (1 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
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GRB2 antibody was raised against purified recombinant human Grb2 protein.
Recognizes a protein with Mr24-25kD, identified as Grb2 (growth-factor receptor-bound protein 2), also known as ASH (abundant Src homology). Grb2 deletion studies indicate that the epitope for this antibody maps to amino acid residues 54-62. No cross-reactivity with other SH2/SH3 signaling intermediates. Species Reactivity: Human, bovine, mouse, rat and Xenopus.
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Immunofluorescence: 1.0 ug/ml with an incubation of 2 hours at RT. Immunohistochemistry: 1-2 ug/ml with an incubation of 30 minutes at RT. (on acetone-fixed frozen and formalin-fixed/paraffin-embedded tissue sections. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.). Positive control: BALB 3T3 or NIH 3T3 cells, kidney or tonsil.
PBS, pH 7.4, 15 mM Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.