Human, Mouse, Rat, Bovine, Xenopus
(tested or 100% immunogen sequence identity)
Also available conjugated with AP
Protein G purified
- IHC - Paraffin
- IHC - Frozen
- Immunofluorescence (1 µg/ml)
- Western blot
- (applications tested for the base form of this product only)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
GRB2 antibody was raised against purified recombinant human Grb2 protein.
Recognizes a protein with Mr24-25kD, identified as Grb2 (growth-factor receptor-bound protein 2), also known as ASH (abundant Src homology). Grb2 deletion studies indicate that the epitope for this antibody maps to amino acid residues 54-62. No cross-reactivity with other SH2/SH3 signaling intermediates. Species Reactivity: Human, bovine, mouse, rat and Xenopus.
Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry. Immunofluorescence: 1.0 ug/ml with an incubation of 2 hours at RT. Immunohistochemistry: 1-2 ug/ml with an incubation of 30 minutes at RT. (on acetone-fixed frozen and formalin-fixed/paraffin-embedded tissue sections. Staining of formalin/paraffin tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.). Positive control: BALB 3T3 or NIH 3T3 cells, kidney or tonsil.
PBS, pH 7.4, 15 mM Sodium Azide, 0.2% BSA
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
For research use only.