Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed in endothelial cells, cultured fibroblasts, and osteosarcoma cells.
Western blotting total cellular protein from cultured rat aortic smooth muscle cells was prepared and analyze. The protein was transferred to a PVDF membrane, blocked 1XTBS, 0.1% tween 20/ 5%NFDM, probed with 5 g/ml of pre-immune serum 5 ug of S125P antibody (a 3 second exposure is shown). The blot was then washed and probed with monoclonal anti-sheep IgG coupled to HRP 1:10,000, and detected with luminol.