Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Rat Monoclonal [clone RB6-8C5] (IgG2b) to Mouse Gr-1 Antigen
IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry (applications tested for the base form of this product only)
Mouse Gr-1 Antigen
Mouse (tested or 100% immunogen sequence identity)
IgG2b Monoclonal [RB6-8C5]
Protein G purified
IHC - Frozen
Flow Cytometry (1:100 - 1:200)
(applications tested for the base form of this product only)
Specificity and Use
Gr-1 Antigen antibody was raised against normal murine bone marrow cells.
Recognizes the mouse Gr-1 antigen, a 21-25kD cell surface protein that belongs to the Ly-6 family of proteins. Clone Reacts predominantly with the Ly-6G protein but weaker reactivity with the Ly-6C protein has been demonstrated. The Gr-1 antigen is primarily a marker of myeloid differentiation. In the bone marrow the level of Gr-1 expression is low on immature myeloblasts and increases as the myeloid cells mature to granulocytes. Gr-1 is also expressed on macrophages and transiently on differentiating monocytes. Expression of Gr-1 on a subpopulation of lymphocytes has also been reported. Clone RB6-8C5 has been reported to deplete mouse mature neutrophils in vivo. XXX recommend the use of MCA2387EL for functional studies.
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. Method sheets are available on request.
PBS, pH 7.4, 0.09% sodium azide.
+4°C or -20°C, Avoid repeated freezing and thawing.