G11 Alpha antibody LS-C24022 is an unconjugated rabbit polyclonal antibody to G11 Alpha (GQ Alpha) from human, mouse, rat and other species. Validated for IP and WB.
Human GQ Alpha / G11 Alpha
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Goat, Hamster, Horse, Pig, Rabbit, Chicken, Xenopus, Zebrafish
(tested or 100% immunogen sequence identity)
- Western blot
- Immunoprecipitation (1:500 - 1:2000)
KLH-conjugated, synthetic peptide (QLNLKEYNLV) corresponding to amino acids 350- 359 of G11 alpha or 344-353 of Gq alpha. This sequence is highly conserved among species. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Goat, Hamster, Elephant, Panda, Bat, Bovine, Dog, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Lizard, Xenopus, Stickleback, Pufferfish, Zebrafish, Blood fluke, Sea anemone, Tick (100%); Water flea (90%).
Specific for human Gq alpha and G11 alpha. Does not recognize other non-pertussis toxin-sensitive G proteins. Species cross-reactivity: Mouse and human.
Suitable for use in Western Blot analysis and Immunoprecipitation. Immunoprecipitation: 1:500-1:2000 (detects Gq alpha and G11 alpha in 20 ug of mouse brain microsomes and membranes; detected Gq/11 a in 10 ug of mouse 3T3/NIH fibroblast soluble plasma membranes.
PBS, pH 7.4, 0.09% Sodium Azide
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles.
For research use only.