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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPT / Alanine Transaminase Antibody (aa86‑112) LS‑C100675
Alanine Transaminase antibody LS-C100675 is an unconjugated rabbit polyclonal antibody to human Alanine Transaminase (GPT). Validated for Flow, IHC and WB.
Catalog
Size
Price
LS-C100675-400
400 µl
Unavailable

Popular GPT / Alanine Transaminase Products

GPT / ALT1 antibody LS-C80760 was used in IHC to stain formalin-fixed, paraffin-embedded human muscle.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Pig
Applications: IHC, IHC - Paraffin, Western blot
Lane A: Marker. Lane B: Transfected 293T cell lysate. Antibody concentration: 1.25 ug/ml. Gel concentration: 12%.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Dog, Hamster, Horse, Rabbit, Zebrafish
Applications: Western blot
Western blot of recombinant GPT / Alanine Transaminase.
Species: Human
Applications: IHC, Western blot
Human Liver: Formalin-Fixed, Paraffin-Embedded (FFPE).  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

Alanine Transaminase antibody LS-C100675 is an unconjugated rabbit polyclonal antibody to human Alanine Transaminase (GPT). Validated for Flow, IHC and WB.
About GPT / Alanine Transaminase
GPT / Alanine Transaminase is cytosolic alanine aminotransaminase 1 (ALT1); also known as glutamate-pyruvate transaminase 1. This enzyme catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. P24298 NM_005309 NP_005300.1

GPT Antibody, AAT1 Antibody, ALT1 Antibody, Alanine aminotransferase Antibody, Alanine aminotransferase 1 Antibody, GPT 1 Antibody, GPT1 Antibody


Specifications

Target
Human GPT / Alanine Transaminase
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:10 - 1:50)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Failed Applications
  • IHC - Paraffin
Blocking Peptide
LS-E8988 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C100675. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa86-112
Specificity
This GPT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-112 amino acids from the N-terminal region of human GPT.
Presentation
PBS, 0.09% sodium azide
Storage
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.
Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.
Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.
Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunohistochemistry

Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Formalin-fixed and paraffin-embedded human hepatocarcinoma with GPT Antibody (N-term P101), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Western blot

Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.
Western blot of GPT antibody (N-term P101) in HL60 cell line lysates (35 ug/lane). GPT (arrow) was detected using the purified antibody.

Flow Cytometry

Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Flow cytometric of 293 cells using GPT Antibody (N-term P101)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.


Requested From: 
Date Requested: 6/18/2018

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