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| Catalog Number | Size | Price |
|---|---|---|
| LS-A538-50 | 50 µg (1 mg/ml) | $440 |
IHC‑plus™ Polyclonal Rabbit anti‑Human GPRC5C Antibody (C‑Terminus, IHC) LS‑A538
IHC‑plus™ Polyclonal Rabbit anti‑Human GPRC5C Antibody (C‑Terminus, IHC) LS‑A538
Antibody:
GPRC5C Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Bat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified
Overview
Antibody:
GPRC5C Rabbit anti-Human Polyclonal (C-Terminus) Antibody
Application:
IHC, IHC-P, ELISA
Reactivity:
Human, Monkey, Bat, Bovine, Dog, Horse
Format:
Unconjugated, Unmodified
Specifications
Description
GPRC5C antibody LS-A538 is an unconjugated rabbit polyclonal antibody to GPRC5C (C-Terminus) from human. It is reactive with human, bat, bovine and other species. Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Host
Rabbit
Reactivity
Human, Monkey, Bat, Bovine, Dog, Horse
(tested or 100% immunogen sequence identity)
Predicted
Mouse, Rat, Hamster, Rabbit, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic 19 amino acid peptide from C-Terminus cytoplasmic domain of human GPRC5C. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Horse (100%); Mouse, Rat, Hamster, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard (95%); Panda (84%).
Epitope
C-Terminus
Specificity
Human GPRC5C. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPRC5B (47%).
Applications
- IHC
- IHC - Paraffin (10 µg/ml)
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: The unconjugated form was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for the unconjugated form was determined to be 10 ug/ml.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About GPRC5C
Validation
Anti-GPRC5C antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPRC5C antibody IHC of human adrenal. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
See More About...
LSBio Ratings
IHC-plus™ GPRC5C Antibody (C-Terminus) for IHC, ELISA LS-A538 has an LSBio Rating of
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (0)
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, ELISA
Species:
Human, Monkey, Dog, Horse
Applications:
IHC, IHC - Paraffin
Species:
Human, Monkey, Mouse, Rat
Applications:
Immunofluorescence, Western blot, ELISA
Reactivity:
Mouse
Range:
78-5000 pg/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/19/2024
Date Requested: 4/19/2024
