Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Monkey, Bat, Bovine, Dog, Horse (tested or 100% immunogen sequence identity)
Mouse, Rat, Hamster, Rabbit, Chicken (at least 90% immunogen sequence identity)
IHC - Paraffin (10 µg/ml), ELISA
Specificity and Use
GPRC5C antibody was raised against synthetic 19 amino acid peptide from C-terminal cytoplasmic domain of human GPRC5C. Percent identity with other species by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Dog, Bat, Bovine, Elephant, Horse (100%); Mouse, Rat, Hamster, Rabbit, Opossum, Turkey, Chicken, Platypus, Lizard (95%); Panda (84%).
Human GPRC5C. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except GPRC5B (47%).
Immunohistochemistry: LS-A538 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A538 was determined to be 10 ug/ml.
PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
GPRC5C is a member of the type 3 G protein-coupled receptor family. Members of this superfamily are characterized by a signature 7-transmembrane domain motif. The specific function of this protein is unknown; however, this protein may mediate the cellular effects of retinoic acid on the G protein signal transduction cascade. Two transcript variants encoding different isoforms have been found for this gene.