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Anti-GPR49 / LGR5 Antibody (aa689-719) LS-C98619


Wt. Vol. Conc. Price
- 400 µl - $305
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)

Most Popular GPR49 / LGR5 Antibodies

Anti-GPR49 / LGR5 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A1236
Rabbit Polyclonal to Human GPR49 / LGR5
IHC - Paraffin
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human GPR49 / LGR5
Immunofluorescence, Western blot, Flow Cytometry


Human GPR49 / LGR5
Human (tested or 100% immunogen sequence identity)
Immunoaffinity purified


  • Immunofluorescence (1:100)
  • Western blot (1:1000)
  • Flow Cytometry (1:10 - 1:50)

  • IHC - Paraffin

Specificity and Use

GPR49 / LGR5 antibody was raised against kLH-conjugated synthetic peptide between amino acids 689-719 from human LGR5.
LS-E7062 - Lyophilized - 100 µg - $145.00


PBS, 0.09% sodium azide
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.
For research use only.

About GPR49 / LGR5

O75473 NM_003667 NP_003658.1

LGR5 Antibody, FEX Antibody, G-protein coupled receptor 49 Antibody, G protein-coupled receptor 49 Antibody, GPR67 Antibody, GRP49 Antibody, GPR49 Antibody, HG38 Antibody, G-protein coupled receptor 67 Antibody

Receptor for R-spondins that potentiates the canonical Wnt signaling pathway and acts as a stem cell marker of the intestinal epithelium and the hair follicle. Upon binding to R-spondins (RSPO1, RSPO2, RSPO3 or RSPO4), associates with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes.


Fluorescent confocal image of HeLa cells stained with LGR5 (loop2) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated LGR5 (loop2) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the LGR5 immunosignal to the nucleus and cytoplasm, supported by Human Protein Atlas Data (

Western blot

Western blot
Western blot of anti-LGR5/GPR49 Antibody (loop2) in mouse cerebellum tissue lysates (35 ug/lane). LGR5/GPR49(arrow) was detected using the purified antibody.

Western blot

Western blot
LGR5/GPR49 Antibody (loop2) western blot of HepG2 cell line lysates (35 ug/lane). The LGR5/GPR49 antibody detected the LGR5/GPR49 protein (arrow).

Flow Cytometry

Flow Cytometry
Flow cytometric of HepG2 cells using LGR5/GPR49 Antibody (loop2) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Requested From: United States
Date Requested: 10/23/2016

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