Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
GPR3 has been identified as a constitutively active lysophospholipid/lysosphingolipid receptor. GPR3 receptor is a link in communication between the somatic cells and oocyte of the ovarian follicle where GPR3 maintains meiotic arrest in mouse oocytes. Oocytes from Gpr3 knockout mice resume meiosis within antral follicles, independently of an increase in luteinizing hormone, and this phenotype Mice deficient in GPR3 exhibit premature ovarian failure due to early oocyte aging (Ledent et al.
Anti-GPR3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4335 concentration 5 ug/ml.
Anti-GPR3 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4335 concentration 5 ug/ml.
Western blot of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody clone 3B4-G3.
GPR3 monoclonal antibody, clone 3B4-G3 Western blot of GPR3 expression in Jurkat.
Enzyme-Linked Immunosorbent Assay
Detection limit for recombinant GST tagged GPR3 is approximately 0.03 ng/ml as a capture antibody.
Western blot of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody, clone 3B4-G3. GAPDH ( 36.1 kD ) used as specificity a.