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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR183 / EBI2 Antibody (Cytoplasmic Domain) IHC‑plus™ LS‑A46 Best Seller
EBI2 antibody LS-A46 is an unconjugated rabbit polyclonal antibody to EBI2 (GPR183) from human, mouse and rat. Validated for IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A46-50
50 µg (1 mg/ml)
$425
In Stock for Immediate Shipment

Popular GPR183 / EBI2 Products

GPR183 / EBI2 antibody Western Blot of 293T.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Horse, Pig, Rabbit
Applications: Western blot
IHC of EBI2 in formalin-fixed, paraffin-embedded human stomach tumor tissue using an isotype control (top left) and LS-C148722 (bottom left, right) at 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Anti-GPR183 / EBI2 antibody LS-A48 IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human
Applications: IHC, IHC - Paraffin
Anti-GPR183 / EBI2 antibody LS-A45 IHC of human peripheral blood leukocytes. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Species: Human, Monkey
Applications: IHC, IHC - Paraffin

Product Description

EBI2 antibody LS-A46 is an unconjugated rabbit polyclonal antibody to EBI2 (GPR183) from human, mouse and rat. Validated for IHC. Tested on 20 paraffin-embedded human tissues.

Specifications

Target
Human GPR183 / EBI2
Synonyms
GPR183, EBI 2, EBI2, EBV-induced gene 2, G protein-coupled receptor 183, G-protein coupled receptor 183
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Predicted
Monkey, Bovine, Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (4 µg/ml)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR183 / EBI2 antibody was raised against synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human GPR183 / EBI2. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Mouse, Rat (100%); Marmoset, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (95%); Platypus (90%); Hamster, Bat, Chicken (85%).
Blocking Peptide
LS-E28980 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A46. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
Cytoplasmic Domain
Specificity
Human GPR183 / EBI2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Presentation
PBS, 0.1% Sodium Azide
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About GPR183 / EBI2
Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). P32249 NM_004951 NP_004942.1


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Images

Immunohistochemistry

Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR183 / EBI2 antibody LS-A46 IHC of human lymph node. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: United States
Date Requested: 10/16/2018

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