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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR183 / EBI2 Antibody (aa140‑149) LS‑C154806
Antibody
EBI2 antibody LS-C154806 is an unconjugated goat polyclonal antibody to EBI2 (GPR183) from human and bovine. Validated for Peptide-ELISA.
Catalog
Size
Price
LS-C154806-100
100 µg (0.5 mg/ml)
Unavailable

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GPR183 / EBI2 antibody Western Blot of 293T.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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Product Description

EBI2 antibody LS-C154806 is an unconjugated goat polyclonal antibody to EBI2 (GPR183) from human and bovine. Validated for Peptide-ELISA.
About GPR183 / EBI2
Receptor for oxysterol 7-alpha,25-dihydroxycholesterol (7-alpha,25-OHC) and other related oxysterols. Binding of 7-alpha,25-OHC mediates the correct localization of B-cells during humoral immune responses. Promotes activated B-cell localization in the outer follicle and interfollicular regions. Its specific expression during B-cell maturation helps position B-cells appropriately for mounting T-dependent antibody responses (By similarity). P32249 NM_004951 NP_004942.1

GPR183 Antibody, EBI 2 Antibody, EBI2 Antibody, EBV-induced gene 2 Antibody, G protein-coupled receptor 183 Antibody, G-protein coupled receptor 183 Antibody


Specifications

Target
Human GPR183 / EBI2
Host
Goat
Reactivity
Human, Bovine (tested or 100% immunogen sequence identity)
Predicted
Monkey, Mouse, Rat, Bat, Dog, Hamster, Horse (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Immunogen
GPR183 / EBI2 antibody was raised against synthetic peptide C-NKIKRIEHAK from an internal region of human GPR183 / EBI2 (NP_004942.1). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Monkey, Bovine, Porcine (100%); Marmoset, Mouse, Rat, Dog, Bat, Hamster, Elephant, Horse (90%); Rabbit, Opossum (80%).
Blocking Peptide
LS-E27547 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C154806. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa140-149
Specificity
Human GPR183 / EBI2.
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Preliminary experiments gave an approx 60kD band in lysates of cell line Daudi after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 41.2kD according to NP_004942.1. The 60kD band was successfully blocked by incubation with the immunizing peptide.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Requested From: 
Date Requested: 6/18/2018

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