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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPR132 / G2A Antibody (C‑Terminus) IHC‑plus™ LS‑A3701 Best Seller
G2A antibody LS-A3701 is an unconjugated rabbit polyclonal antibody to human G2A (GPR132). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-A3701-50
50 µg (1 mg/ml)
Unavailable
In Stock for Immediate Shipment

Popular GPR132 / G2A Products

Anti-GPR132 / G2A antibody LS-A3702 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
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Western blot analysis of lysates from Jurkat cells, using GPR132 Antibody. The lane on the right is blocked with the synthesized peptide.
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Product Description

G2A antibody LS-A3701 is an unconjugated rabbit polyclonal antibody to human G2A (GPR132). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
About GPR132 / G2A
May be a receptor for oxidized free fatty acids derived from linoleic and arachidonic acids such as 9-hydroxyoctadecadienoic acid (9-HODE). Activates a G alpha protein, most likely G alpha(q). May be involved in apoptosis. Functions at the G2/M checkpoint to delay mitosis. May function as a sensor that monitors the oxidative states and mediates appropriate cellular responses such as secretion of paracrine signals and attenuation of proliferation. Q9UNW8 NM_013345 NP_037477.1

GPR132 Antibody, G2A Antibody, G protein-coupled receptor 132 Antibody, G protein-coupled receptor G2A Antibody, G2 accumulation protein Antibody, G2a receptor Antibody


Specifications

Target
Human GPR132 / G2A
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with AP, APC, Biotin, FITC, HRP, PE.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (12 µg/ml)
  • ELISA
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
GPR132 / G2A antibody was raised against synthetic 18 amino acid peptide from C-terminus of human GPR132 / G2A. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Marmoset (89%); Gibbon, Monkey (83%).
Blocking Peptide
LS-E28797 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A3701. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
C-Terminus
Specificity
Human GPR132 / G2A. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
Usage
Immunohistochemistry: LS-A3701 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A3701 was determined to be 11 ug/ml.
Presentation
PBS, less than 0.1% sodium azide.
Storage
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images

Immunohistochemistry

Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
Anti-GPR132 / G2A antibody LS-A3701 IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 7/19/2018

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