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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GPNMB / Osteoactivin Antibody (aa31‑260, clone 7C10E5) LS‑C191872
Osteoactivin antibody LS-C191872 is an unconjugated mouse monoclonal antibody to human Osteoactivin (GPNMB). Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C191872-100
100 µg (1 mg/ml)
Unavailable

Popular GPNMB / Osteoactivin Products

Anti-GPNMB antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B6406 concentration 5 ug/ml.
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Anti-GPNMB antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Mouse, Bovine, Hamster, Horse, Rabbit
Applications: IHC, IHC - Paraffin, Western blot
IHC of paraffin-embedded Human tonsil using anti-GPNMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120°C for 3min).
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Antibody
Species: Mouse
Applications: ELISA

Product Description

Osteoactivin antibody LS-C191872 is an unconjugated mouse monoclonal antibody to human Osteoactivin (GPNMB). Validated for ELISA, IHC and WB.
About GPNMB / Osteoactivin
GPNMB / Osteoactivin is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene. Q14956 NM_002510 NP_002501.1

GPNMB Antibody, Glycoprotein nmb-like protein Antibody, HGFIN Antibody, Glycoprotein NMB Antibody, Osteoactivin Antibody, Transmembrane glycoprotein NMB Antibody


Specifications

Target
Human GPNMB / Osteoactivin
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal [7C10E5]
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:200 - 1:1000)
  • Western blot (1:100 - 1:500)
  • ELISA (1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Purified recombinant fragment of human GPNMB (AA: 31-260) expressed in E. Coli.
Epitope
aa31-260
Specificity
Human
Presentation
Purified antibody in PBS containing 0.05% sodium azide
Storage
Short term: 4°C; Long term: -20°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Western blot

Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.
Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.

Western blot

Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)

Western blot

Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Western blot

Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.
Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.

Western blot

Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)

Western blot

Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Western blot

Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.
Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.

Western blot

Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)

Western blot

Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Immunohistochemistry

IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded breast cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Immunohistochemistry

IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.
IHC of paraffin-embedded esophagus cancer tissues using GPNMB mouse monoclonal antibody with DAB staining.

Western blot

Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.
Western blot using GPNMB mouse monoclonal antibody against PANC1 (1) and PC-3 (2) cell lysate.

Western blot

Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)
Western blot using GPNMB monoclonal antibody against human GPNMB recombinant protein. (Expected MW is 47.0 kDa)

Western blot

Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.
Western blot using GPNMB monoclonal antibody against HEK293 (1) and GPNMB (AA: 31-260)-hIgGFc transfected HEK293 (2) cell lysate.

Enzyme-Linked Immunosorbent Assay

Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);


Requested From: 
Date Requested: 6/23/2018

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