LSBio The Immunohistochemistry Antibody Company
Products
  • Antibodies
  • All Antibodies
  • Primary Antibodies
  • Secondary Antibodies
  • IHC-plus Antibodies
  • Isotype Control Antibodies
  • ELISA Kits
  • All Kits
  • Sandwich ELISA Kits
  • Competitive EIA Kits
  • Cell-Based ELISA Kits
  • DNA-Binding ELISA Kits
  • Direct ELISA Kits
  • Functional ELISA Kits
  • Phospho-Specific ELISA Kits
  • Custom ELISA Kits
  • Proteins
  • All Proteins
  • Recombinant Proteins
  • Native Proteins
  • Over-expression Lysates
  • Cell and Tissue Lysates
  • Bio-active Proteins
  • Animal-free Proteins
  • Immunohistochemistry
  • Comprehensive IHC Reports
  • Custom IHC Services
  • TCR Screening Services
  • IHC-plus Antibodies
  • Other
  • Blocking Peptides
Services
  • Immunohistochemistry Services

  • Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

  • TCR Screening Services

  • Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Have a question? CustomerSupport@LSBio.com
Resources
  • Purchasing
  • Product Ordering Terms and Conditions
  • Holiday Schedule
  • About the LSBio Guarantee
  • About Our Rewards Program
  • Reference Material
  • About LSBio (LifeSpan BioSciences Inc.)
  • About Our 2-million Specimen Tissue Bank
  • About Our IHC Validation Process
  • About Our IHC-plus™ Protocol
  • Publications
  • Secure Logins
  • Login to Our Secure FTP Site
  • Login to Our IHC Database
Distributors
Contact Us

LSBio
2401 Fourth Avenue Suite 900
Seattle WA 98121

Phone: 866-819-4732 (Toll Free North America)
206-374-1102 (International)

Fax: 866-206-6909 (Toll Free North America)
206-577-4565 (International)
How to Buy - Details about how to buy our products.

Orders@LSBio.com - To submit a new order.

Customer.Support@LSBio.com - To submit questions about existing orders, pricing, availability, bulk quotes, proforma invoice requests, or other billing issues.

Technical.Support@LSBio.com - To request technical information requests about an LSBio product or its application.

Sales@LSBio.com - To request information about fee-for-service contract IHC studies, IHC reports, distribution agreements, or general business development.

Worldwide Distributors List - To find your local distributor if you're not within the United States.
Quick Order ▾
View Cart

Anti-GPER1 / GPR30 Antibody (Extracellular Domain) IHC-plus™ LS-A4271

Note: This antibody replaces LS-C66914

Ordering

Wt. Vol. Conc. Price
50 µg 50 µl 1 mg/ml $395
Inquire for larger quantities
Same day shipping In Stock - Same day shipping

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

100% Guaranteed 100% Guaranteed In Stock In Stock Publications Publications (8)
Rabbit Polyclonal to Human GPER1 / GPR30
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated

Details

Human GPER1 / GPR30
Rabbit
Human, Monkey (tested or 100% immunogen sequence identity)
Mouse, Rat, Dog, Hamster (at least 90% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (6.4 µg/ml), Immunofluorescence, Western blot

Specificity and Use

GPER1 / GPR30 antibody was raised against synthetic 16 amino acid peptide from 3rd extracellular domain of human GPR30. Percent identity with other species by BLAST analysis: Human, Gorilla, Monkey, Marmoset (100%); Mouse, Rat, Hamster, Elephant, Dog, Opossum (94%); Panda, Bat, Horse, Rabbit, Platypus (88%).
LS-E28932 - Liquid - 50 µg 1 mg/ml - $145.00
Extracellular Domain
Human GPR30. BLAST analysis of the peptide immunogen showed no homology with other human proteins.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

Publications (8)

1
Identification of the estrogen receptor GPER in neoplastic and non-neoplastic human testes. Rago V, Romeo F, Giordano F, Maggiolini M, Carpino A. Reproductive biology and endocrinology : RB&E. 2011 9:135. (WB, IHC-P; Human) [Full Text Article] [PubMed:21974818] [PMC:PMC3200171]
2
Nuclear alternate estrogen receptor GPR30 mediates 17beta-estradiol-induced gene expression and migration in breast cancer-associated fibroblasts. Madeo A, Maggiolini M. Cancer research. 2010 Jul;70:6036-46. [Full Text Article] [PubMed:20551055]
3
Identification of G protein-coupled estrogen receptor in human and pig spermatozoa. Rago V, Giordano F, Brunelli E, Zito D, Aquila S, Carpino A. Journal of anatomy. 2014 Jun;224:732-6. [Full Text Article] [PubMed:24697543]
4
Expression pattern of G protein-coupled receptor 30 in LacZ reporter mice. Isensee J, Meoli L, Zazzu V, Nabzdyk C, Witt H, Soewarto D, Effertz K, Fuchs H, Gailus-Durner V, Busch D, Adler T, de Angelis MH, Irgang M, Otto C, Noppinger PR. Endocrinology. 2009 Apr;150:1722-30. [Full Text Article] [PubMed:19095739]
5
G protein-coupled receptor 30 expression is up-regulated by EGF and TGF alpha in estrogen receptor alpha-positive cancer cells. Vivacqua A, Lappano R, De Marco P, Sisci D, Aquila S, De Amicis F, Fuqua SA, And[Character f2] S, Maggiolini M. Molecular endocrinology (Baltimore, Md.). 2009 Nov;23:1815-26. [Full Text Article] [PubMed:19749156]
6
c-Jun activation is required for 4-hydroxytamoxifen-induced cell death in breast cancer cells. Madeo A, Vinciguerra M, Lappano R, Galgani M, Gasperi-Campani A, Maggiolini M, Musti AM. Oncogene. 2010 Feb;29:978-91. [Full Text Article] [PubMed:19935718]
7
Stallion spermatozoa: putative target of estrogens; presence of the estrogen receptors ESR1, ESR2 and identification of the estrogen-membrane receptor GPER. Arkoun B, Gautier C, Delalande C, Barrier-Battut I, Gu[Character e9]non I, Goux D, Boura[Character ef]ma-Lelong H. General and comparative endocrinology. 2014 May;200:35-43. [Full Text Article] [PubMed:24607572]
8
Estriol acts as a GPR30 antagonist in estrogen receptor-negative breast cancer cells. Lappano R, Rosano C, De Marco P, De Francesco EM, Pezzi V, Maggiolini M. Molecular and cellular endocrinology. 2010 May;320:162-70. [Full Text Article] [PubMed:20138962]
more

About GPER1 / GPR30

Q99527 NM_001505 NP_001496.1

GPER1 Antibody, CMKRL2 Antibody, CEPR Antibody, FEG-1 Antibody, G-protein coupled receptor 30 Antibody, G protein-coupled receptor 30 Antibody, GPCR-Br Antibody, GPER Antibody, Heptahelix receptor Antibody, LERGU Antibody, LyGPR Antibody, MER Antibody, GPR30 Antibody, IL8-related receptor DRY12 Antibody, LERGU2 Antibody, Chemokine receptor-like 2 Antibody, DRY12 Antibody, Membrane estrogen receptor Antibody

G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK.

Immunohistochemistry

Immunohistochemistry
Anti-GPR30 antibody LS-A4271 IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.
 

Requested From: United States
Date Requested: 9/29/2016

Get Social With Us! Follow us on Facebook Follow us on Google+ Follow us on LinkedIn
Copyright © 2016 LifeSpan BioSciences, Inc. All Rights Reserved Privacy Policy
Catalog Number