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Anti-GPER1 / GPR30 Antibody (aa1-62) LS-C149963

Ordering

Wt. Vol. Conc. Price
100 µg 500 µl 0.2 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GPER1 / GPR30 Antibodies

Anti-GPER1 / GPR30 Antibody (Extracellular Domain) IHC-plus™ LS-A1184
Rabbit Polyclonal to Human GPER1 / GPR30
Human
IHC - Paraffin, Immunofluorescence
Unconjugated
Immunohistochemistry Image
Anti-GPER1 / GPR30 Antibody (Extracellular Domain) IHC-plus™ LS-A4271
Rabbit Polyclonal to Human GPER1 / GPR30
Human, Monkey
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GPER1 / GPR30 Antibody (C-Terminus) IHC-plus™ LS-A4272
Rabbit Polyclonal to Human GPER1 / GPR30
Human, Monkey, Dog
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal (IgG) to Human GPER1 / GPR30
Human
IHC - Paraffin, Western blot
Unconjugated

Details

Human GPER1 / GPR30
Goat
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

GPER1 / GPR30 antibody was raised against e. coli-derived recombinant human GPER Met1-Ser62 Accession # Q99527.
aa1-62
Detects human GPER in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human GPR144.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
500 µl Reconstitute with sterile PBS.
Store at -20°C prior to reconstitution. After reconstitution aliquot and freeze at-20°C. Avoid frequent freeze thaws.
For research use only.

About GPER1 / GPR30

Q99527 NM_001505 NP_001496.1

GPER1 Antibody, CMKRL2 Antibody, CEPR Antibody, FEG-1 Antibody, G-protein coupled receptor 30 Antibody, G protein-coupled receptor 30 Antibody, GPCR-Br Antibody, GPER Antibody, Heptahelix receptor Antibody, LERGU Antibody, LyGPR Antibody, MER Antibody, GPR30 Antibody, IL8-related receptor DRY12 Antibody, LERGU2 Antibody, Chemokine receptor-like 2 Antibody, DRY12 Antibody, Membrane estrogen receptor Antibody

G-protein coupled estrogen receptor that binds to 17-beta-estradiol (E2) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. Stimulates cAMP production, calcium mobilization and tyrosine kinase Src inducing the release of heparin-bound epidermal growth factor (HB-EGF) and subsequent transactivation of the epidermal growth factor receptor (EGFR), activating downstream signaling pathways such as PI3K/Akt and ERK/MAPK.

Immunohistochemistry

Immunohistochemistry
GPER in Human Brain. GPER was detected in immersion fixed paraffin-embedded sections of human brain (hypothalamus) using Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Tissue was stained using Anti-Goat HRP-DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies.

Western blot

Western blot
Detection of Human GPER by Western Blot. Western blot shows lysates of human brain cortex tissue. PVDF membrane was probed with 1 ug/ml of Goat Anti-Human GPER Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. A specific band was detected for GPER at approximately 54 kD (as indicated). This experiment was conducted under reducing conditions.

Requested From: United States
Date Requested: 12/3/2016

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