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Anti-GPCR6 / GPR101 Antibody (Cytoplasmic Domain) IHC-plus™ LS-A1836

Catalog Size Price
LS-A1836-50 50 µg (1 mg/ml) Unavailable
Same day shipping In Stock - Same day shipping

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Anti-GPCR6 / GPR101 Antibody (aa430-510) LS-C383734
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Anti-GPCR6 / GPR101 Antibody (C-Terminus) LS-C413112
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100% Guaranteed In Stock
Rabbit Polyclonal to Human GPCR6 / GPR101
Human
IHC - Paraffin
Unconjugated

Details

Human GPCR6 / GPR101
Rabbit
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

IHC - Paraffin (7.3 µg/ml)

Specificity and Use

GPCR6 / GPR101 antibody was raised against synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR101. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%); Bovine, Horse, Pig (89%); Bat, Panda, Rabbit (84%).
LS-E28411 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1836. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Cytoplasmic Domain
Human GPR101. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZBTB10 (47%).
Immunohistochemistry: LS-A1836 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1836 was determined to be 7.3 ug/ml.

Packaging

PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.
For research use only.

About GPCR6 / GPR101

Q96P66 NM_054021 NP_473362.1

GPR101 Antibody, G protein-coupled receptor 101 Antibody, GPCR6 Antibody

G protein-coupled receptor GPR101 is an Orphan-U GPCR with an unknown ligand.

Immunohistochemistry

Immunohistochemistry
Anti-GPR101 antibody LS-A1836 IHC of human brain, hypothalamus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Immunohistochemistry
Anti-GPCR6 / GPR101 antibody IHC of human Ovary, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry

Immunohistochemistry
Anti-GPCR6 / GPR101 antibody IHC of human Lung, Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Requested From: 
Date Requested: 10/22/2017

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