Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Rabbit Polyclonal to Human GPCR6 / GPR101
IHC - Paraffin
Human GPCR6 / GPR101
Human (tested or 100% immunogen sequence identity)
Monkey (at least 90% immunogen sequence identity)
IHC - Paraffin (7.3 µg/ml)
Specificity and Use
GPCR6 / GPR101 antibody was raised against synthetic 19 amino acid peptide from 3rd cytoplasmic domain of human GPR101. Percent identity with other species by BLAST analysis: Human, Gorilla (100%); Gibbon, Monkey (95%); Bovine, Horse, Pig (89%); Bat, Panda, Rabbit (84%).
LS-E28411 - Liquid - 50 µg - $145.00
Immunizing peptide used to generate LS-A1836. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Human GPR101. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZBTB10 (47%).
Immunohistochemistry: LS-A1836 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-A1836 was determined to be 7.3 ug/ml.
PBS, less than 0.1% sodium azide.
Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze thaw cycles.