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Anti-GPC3 / Glypican 3 Antibody (aa25-558) LS-C149961

Ordering

Wt. Vol. Conc. Price
100 µg - - $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GPC3 / Glypican 3 Antibodies

Anti-GPC3 / Glypican 3 Antibody (PE) LS-C36515
Mouse Monoclonal (IgG2a) to Human GPC3 / Glypican 3
Human
Flow Cytometry
PE Conjugated
Anti-GPC3 / Glypican 3 Antibody (clone 9C2) LS-C169086
Mouse Monoclonal [clone 9C2] (IgG1) to Human GPC3 / Glypican 3
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GPC3 / Glypican 3 Antibody (aa324-373) IHC-plus™ LS-B11249
Rabbit Polyclonal (IgG) to Human GPC3 / Glypican 3
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Sheep Polyclonal (IgG) to Human GPC3 / Glypican 3
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated

Details

Human GPC3 / Glypican 3
Sheep
Human (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 - 15 µg/ml)
  • Western blot (0.1 µg/ml)
  • Flow Cytometry

Specificity and Use

GPC3 / Glypican 3 antibody was raised against mouse myeloma cell line NS0-derived recombinant human Glypican 3 Gln25-Val558 Accession # P51654.1.
aa25-558
Detects human Glypican 3 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human.
Western Blot, 0.1 µg/ml, Recombinant Human Glypican 3. Flow Cytometry, 2.5 µg/106 cells, HepG2 human hepatocellular carcinoma cell line. Immunohistochemistry, 5-15 µg/ml.

Packaging

Lyophilized from 0.2 um filtered solution in PBS, trehalose
Sterile PBS. Resuspend to 500 ul
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About GPC3 / Glypican 3

P51654 NM_004484 NP_004475.1

GPC3 Antibody, DGSX Antibody, Glypican proteoglycan 3 Antibody, Glypican 3 Antibody, Glypican-3 Antibody, GTR2-2 Antibody, Intestinal protein OCI-5 Antibody, SGB Antibody, SGBS1 Antibody, SGBS Antibody, MXR7 Antibody, OCI-5 Antibody, OCI5 Antibody, SDYS Antibody, Secreted glypican-3 Antibody, Heparan sulphate proteoglycan Antibody

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation.

Immunohistochemistry

Immunohistochemistry
Glypican 3 in Human Liver Cancer Tissue. Glypican 3 was detected in immersion fixed paraffin-embedded sections of human liver cancer tissue using Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody at 10 ug/ml overnight at 4°C. Tissue was stained using Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents.

Immunohistochemistry

Immunohistochemistry
Glypican 3 in Human Breast. Glypican 3 was detected in immersion fixed paraffin-embedded sections of human breast using 5 ug/ml Sheep Anti-Human Glypican 3 Antigen Affinity-purified Polyclonal Antibody overnight at 4°C. Tissue was stained with Anti-Sheep HRP-DAB (brown) and counterstained with hematoxylin (blue).

Requested From: United States
Date Requested: 12/10/2016

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