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Catalog Number Size Price
LS-B10598-50 50 µg (1 mg/ml) $515 
GPC1 / Glypican Antibody - Anti-GPC1 / Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
GPC1 / Glypican Antibody - Anti-Glypican-1 Antibody - Western Blot. Western blot of protein A purified anti-Glypican-1 antibody shows detection of exogenous glypican-1 in 50 ug 293T whole cell lysate (lane 2). Minor bands may represent post translational modifications of glypican-1. The blot was incubated overnight with primary antibody at a 1:1000 dilution followed by washes and exposure to the secondary antibody HRP Gt-a-Rabbit IgG diluted 1:5000 in 5% BLOTTO at 4C. Lane 1: untransfected 293T cell lysate. Personal Communication, Caterina Bianco, CCR-NCI, Bethesda, MD.
GPC1 / Glypican Antibody - Anti-GPC1 / Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
GPC1 / Glypican Antibody - Anti-Glypican-1 Antibody - Western Blot. Western blot of protein A purified anti-Glypican-1 antibody shows detection of exogenous glypican-1 in 50 ug 293T whole cell lysate (lane 2). Minor bands may represent post translational modifications of glypican-1. The blot was incubated overnight with primary antibody at a 1:1000 dilution followed by washes and exposure to the secondary antibody HRP Gt-a-Rabbit IgG diluted 1:5000 in 5% BLOTTO at 4C. Lane 1: untransfected 293T cell lysate. Personal Communication, Caterina Bianco, CCR-NCI, Bethesda, MD.
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IHC‑plus™ Polyclonal Rabbit anti‑Human GPC1 / Glypican Antibody (Internal, IHC, WB) LS‑B10598

IHC‑plus™ Polyclonal Rabbit anti‑Human GPC1 / Glypican Antibody (Internal, IHC, WB) LS‑B10598

Note: This antibody replaces LS-C154022
Antibody:
GPC1 / Glypican Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$515
LS-B10598-50
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
GPC1 / Glypican Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Glypican antibody LS-B10598 is an unconjugated rabbit polyclonal antibody to Glypican (GPC1) (Internal) from human. It is reactive with human, mouse and rat. Validated for ELISA, IHC and WB. Tested on 20 paraffin-embedded human tissues.
Target
Human GPC1 / Glypican
Synonyms
GPC1 | Glypican 1 | Glypican | Glypican-1 | Glypican proteoglycan 1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Anti-Glypican-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of human glypican-1 protein.
Epitope
Internal
Applications
  • IHC
  • IHC - Paraffin (10 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:10000 - 1:50000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
This protein A purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately ~61 kD in size corresponding to glypican by western blotting in the appropriate cell lysate or extract. The higher molecular weight (110kD) of transfected Fc-glypican compared with the expected MW of glypican is likely due to the presence of the Fc-tag.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPC1 / Glypican
P35052 NM_002081 NP_002072.2

Validation

GPC1 / Glypican Antibody - Anti-GPC1 / Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-GPC1 / Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
Anti-GPC1 / Glypican antibody IHC staining of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.

LSBio Ratings

IHC-plus™ GPC1 / Glypican Antibody (Internal) for IHC, WB/Western, ELISA LS-B10598 has an LSBio Rating of
Laboratory Validation Score (4)

Publications (0)

Customer Reviews (0)


Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 4/18/2024