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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

TCR Screening Services

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

Research Areas
Cell Cycle Pathways
Protein Family And Group
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Catalog Number Size Price
LS-B15164-50 50 µg (0.25 mg/ml) $425 
GPA33 / A33 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPA33 / A33 Antibody - Immunofluorescence staining of MCF-7 cells with GPA33 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
GPA33 / A33 Antibody - Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: GPA33 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 50 kDa
GPA33 / A33 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
GPA33 / A33 Antibody - Immunofluorescence staining of MCF-7 cells with GPA33 Antibody at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
GPA33 / A33 Antibody - Western Blot Positive WB detected in: K562 whole cell lysate, SH-SY5Y whole cell lysate All lanes: GPA33 antibody at 3.2µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 36 kDa Observed band size: 50 kDa
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IHCPlus™ GPA33 / A33 Antibody Polyclonal ELISA,IHC LS‑B15164

IHCPlus™ GPA33 / A33 Antibody Polyclonal ELISA,IHC LS‑B15164

Note: This antibody replaces LS-C371954
Antibody:
Rabbit Polyclonal to Human GPA33 / A33
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:
Price
Catalog Number
$425 
LS-B15164-50
50 µg
0.25 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human GPA33 / A33
Application:
IHC, IHC-P, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Other formats:

Specifications

Description
A33 antibody LS-B15164 is an unconjugated rabbit polyclonal antibody to human A33 (GPA33 ). Validated for ELISA and IHC. Tested on 20 paraffin-embedded human tissues.
Target
Human GPA33 / A33
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated. Also available conjugated with HRP, Biotin, FITC.
Purification
Caprylic acid and ammonium sulfate precipitation
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ELISA
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Recombinant human Cell surface A33 antigen protein.
Specificity
Human GPA33 / A33
Presentation
PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
Storage
Short term: -20°C; Long term: -80°C; Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About GPA33 / A33
Q99795 NM_005814 NP_005805.1

Validation

GPA33 / A33 Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)
Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE)

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Requested From: United States
Date Requested: 12/10/2019