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GOLPH3

golgi phosphoprotein 3 (coat-protein)

Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.

Gene Name: golgi phosphoprotein 3 (coat-protein)
Synonyms: GOLPH3, Coat protein GPP34, Coat-protein, Golgi protein, Golgi phosphoprotein 3, GPP34, Golgi-associated protein, GOPP1, MIDAS
Target Sequences: NM_022130 NP_071413.1 Q9H4A6

Publications (1)

1
Golgi-Related Proteins GOLPH2 (GP73/GOLM1) and GOLPH3 (GOPP1/MIDAS) in Cutaneous Melanoma: Patterns of Expression and Prognostic Significance. Donizy P, Kaczorowski M, Biecek P, Halon A, Szkudlarek T, Matkowski R. International journal of molecular sciences. 2016 17: (WB; Human) [Full Text Article] [PubMed:27706081] [PMC:PMC5085652] Related Antibodies: LS-B5044.

☰ Filters
Antibodies (22)
Proteins (4)
ELISA Kits (3)
Over-Expression Lysate (2)
Primary (22)
Recombinant (2)
Sandwich (3)
GOLPH3 (29)
Human (23)
Mouse (13)
Rat (10)
Bovine (3)
Chimpanzee (3)
Dog (3)
Gibbon (3)
Guinea pig (3)
Horse (3)
Monkey (3)
Pig (3)
Rabbit (3)
Zebrafish (3)
IHC (7)
IHC-P (7)
WB (18)
Flo (2)
ELISA (5)
IF (3)
IP (3)
Peptide-ELISA (1)
rabbit (20)
mouse (2)
Product Group
IHCPlus (2)
IgG (15)
IgG1 (1)
monoclonal mc (1)
polyclonal pc (21)
905CT9.1.1 (1)
96-Well Strip Plate (3)
Biotin Conjugated (2)
FITC Conjugated (2)
HRP Conjugated (2)
Unconjugated (16)
aa114-163 (4)
aa1-30 (1)
aa1-50 (1)
aa25-75 (1)
aa251-279 (1)
No (28)
Yes (1)
Plasma (3)
Serum (3)
GST (1)
Myc-DDK (Flag) (3)
Human (3)
293T Cells (1)
HEK 293 Cells (2)
Wheat Germ Extract (1)
Purified (1)
GOLPH3 Antibody - Anti-GOLPH3 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IF, IHC, IHC-P, WB
Unconjugated
0.05 mg/$545
GOLPH3 Antibody - Anti-GOLPH3 antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 7.5 ug/ml.
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Human
IHC, IHC-P, WB
Unconjugated
50 µl/$460
GOLPH3 Antibody - Formalin-fixed and paraffin-embedded human lung carcinoma with GOLPH3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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GOLPH3 Rabbit anti-Human Polyclonal (aa251-279) Antibody
Mouse, Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
GOLPH3 Antibody - Immunohistochemistry image of paraffin-embedded human prostate cancer at a dilution of 1:100
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IHC, IHC-P, WB
Unconjugated
50 µg/$294; 100 µg/$360
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GOLPH3 Rabbit anti-Human Polyclonal (HRP) Antibody
Human
HRP Conjugated
50 µg/$294; 100 µg/$360
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GOLPH3 Rabbit anti-Human Polyclonal (Biotin) Antibody
Human
Biotin Conjugated
50 µg/$294; 100 µg/$360
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GOLPH3 Rabbit anti-Human Polyclonal (FITC) Antibody
Human
FITC Conjugated
50 µg/$294; 100 µg/$360
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Human
ELISA, IF, IHC, IP, WB
Unconjugated
100 µg/$331
GOLPH3 Antibody - 1:100 staining mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
IF, IHC, Peptide-ELISA, WB
Unconjugated
100 µl/$379; 200 µl/$421
GOLPH3 Antibody - Formalin-fixed and paraffin-embedded human prostate carcinoma reacted with GOLPH3 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
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GOLPH3 Rabbit anti-Human Polyclonal (aa1-30) Antibody
Human
Flo, IHC, IHC-P, WB
Unconjugated
400 µl/$393
GOLPH3 Antibody - IHC analysis of GOLPH3 using anti-GOLPH3 antibody. GOLPH3 was detected in paraffin-embedded section of mouse lung tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-GOLPH3 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC-P, WB
Unconjugated
10 µg/$318; 100 µg/$470
GOLPH3 Antibody - IHC staining of FFPE human lung cancer with GOLPH3 antibody at 2ug/ml. HIER: boil tissue sections in pH6, 10mM citrate buffer, for 10-20 min followed by cooling at RT for 20 min.
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
ELISA, IHC-P, WB
Unconjugated
100 µg/$575
GOLPH3 Antibody - Detection of human and mouse GOLPH3 by western blot. Samples: Whole cell lysate (15 µg) from HeLa, MCF-7, U2OS, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-GOLPH3 antibody used for WB at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.
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GOLPH3 Rabbit anti-Human Polyclonal (aa1-50) Antibody
Mouse, Human
IP, WB
Unconjugated
10 µl/$295
GOLPH3 Antibody - GOLPH3 Antibody western blot of 293,MDA-MB-231 cell line lysates (35 ug/lane). The GOLPH3 antibody detected the GOLPH3 protein (arrow).
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GOLPH3 Mouse anti-Human Monoclonal (905CT9.1.1) Antibody
Human
WB
Unconjugated
400 µl/$393
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
60 µl/$331; 120 µl/$403; 200 µl/$529
GOLPH3 Antibody - GOLPH3 antibody Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GOLPH3 Rabbit anti-Human Polyclonal (aa114-163) Antibody
Human
WB
Unconjugated
100 µl/$424
GOLPH3 Antibody - GOLPH3 antibody Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GOLPH3 Rabbit anti-Human Polyclonal (aa114-163) (HRP) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Human, Monkey, Gibbon, Chimpanzee
WB
HRP Conjugated
100 µl/$469
GOLPH3 Antibody - GOLPH3 antibody Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GOLPH3 Rabbit anti-Human Polyclonal (aa114-163) (Biotin) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Human, Monkey, Gibbon, Chimpanzee
WB
Biotin Conjugated
100 µl/$469
GOLPH3 Antibody - GOLPH3 antibody Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
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GOLPH3 Rabbit anti-Human Polyclonal (aa114-163) (FITC) Antibody
Zebrafish, Pig, Horse, Rabbit, Mouse, Dog, Guinea pig, Bovine, Rat, Human, Monkey, Gibbon, Chimpanzee
WB
FITC Conjugated
100 µl/$469
GOLPH3 Antibody - Western blot analysis of extracts of various cell lines, using GOLPH3 antibody at 1:3000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 15s.
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GOLPH3 Rabbit anti-Human Polyclonal Antibody
Mouse, Rat, Human
WB
Unconjugated
20 µl/$275; 50 µl/$311; 100 µl/$389; 200 µl/$503
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GOLPH3 Rabbit anti-Human Polyclonal (aa25-75) Antibody
Human
IP
Unconjugated
10 µl/$295
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GOLPH3 Mouse anti-Human Polyclonal Antibody
Human
WB
Unconjugated
50 µg/$705
GOLPH3 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Mouse
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
GOLPH3 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Human
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
GOLPH3 ELISA Kit
Select
Sandwich
96-Well Strip Plate
Rat
0.156 - 10 ng/ml
Colorimetric - 450nm (TMB)
Plasma, Serum
1 Plate/$783
Viewing 1-25 of 29 product results



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For RESEARCH USE ONLY. Intended for use by laboratory professionals. Not intended for human diagnostic or therapeutic purposes.

The data on this page has been compiled from LifeSpan internal sources, the National Center for Biotechnology Information (NCBI), and The Universal Protein Resource (UniProt).