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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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GOLPH3 Antibody (aa114‑163) LS‑C145732
GOLPH3 antibody LS-C145732 is an unconjugated rabbit polyclonal antibody to GOLPH3 from human, mouse, rat and other species. Validated for WB.
Catalog
Size
Price
LS-C145732-50
50 µg
Unavailable

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Applications: IHC, Immunofluorescence, Western blot, Immunoprecipitation, ELISA

Product Description

GOLPH3 antibody LS-C145732 is an unconjugated rabbit polyclonal antibody to GOLPH3 from human, mouse, rat and other species. Validated for WB.
About GOLPH3
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Q9H4A6 NM_022130 NP_071413.1

GOLPH3 Antibody, Coat protein GPP34 Antibody, Coat-protein Antibody, Golgi protein Antibody, Golgi phosphoprotein 3 Antibody, GPP34 Antibody, Golgi-associated protein Antibody, GOPP1 Antibody, MIDAS Antibody


Specifications

Target
Human GOLPH3
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)
Predicted
Bat, Chicken (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated. Also available conjugated with Biotin, FITC, HRP.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • Western blot
  • (applications tested for the base form of this product only)
Immunogen
GOLPH3 antibody was raised against synthetic peptide located between aa114-163 of human GOLPH3 (Q9H4A6, NP_071413). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Galago, Marmoset, Mouse, Rat, Elephant, Dog, Bovine, Rabbit, Horse, Pig, Opossum, Guinea pig, Platypus, Zebrafish (100%); Bat, Turkey, Zebra finch, Chicken (92%); Drosophila, Beetle (85%).
Blocking Peptide
LS-E22709 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-C145732. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa114-163
Specificity
Human GOLPH3
Presentation
Lyophilized from PBS with 2% sucrose
Reconstitution
Distilled water
Storage
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Western blot

GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
GOLPH3 antibody LS-C145732 Western blot of Fetal Liver lysate. Antibody concentration 1 ug/ml. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/22/2018

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