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Anti-GNAT3 / Gustducin Antibody (aa304-318) IHC-plus™ LS-B4942

Note: This antibody replaces LS-C87232

Ordering

Wt. Vol. Conc. Price
50 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GNAT3 / Gustducin Antibodies

Anti-GNAT3 / Gustducin Antibody (aa304-318) IHC-plus™ LS-B4942
Goat Polyclonal to Human GNAT3 / Gustducin
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GNAT3 / Gustducin Antibody (aa78-105) LS-C166554
Rabbit Polyclonal to Human GNAT3 / Gustducin
Human
IHC - Paraffin, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image
Anti-GNAT3 / Gustducin Antibody (aa78-105, FITC) LS-C235798
Rabbit Polyclonal (IgG) to Human GNAT3 / Gustducin
Human
IHC, Western blot, Flow Cytometry, ELISA
FITC Conjugated

100% Guaranteed 100% Guaranteed Publications Publications (1)
Goat Polyclonal to Human GNAT3 / Gustducin
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit
IHC - Paraffin, ELISA
Unconjugated

Details

Human GNAT3 / Gustducin
Goat
Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Horse, Pig, Rabbit (tested or 100% immunogen sequence identity)
Bat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • ELISA (1:32000)

Specificity and Use

GNAT3 / Gustducin antibody was raised against synthetic peptide C-KNQFLDLNLKKEDKE from an internal region of human GNAT3 (NP_001095856.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Dog, Bovine, Horse, Rabbit, Pig, Opossum (100%); Bat, Lizard (93%); Platypus (87%); Turkey, Chicken (80%).
LS-E26550 - Lyophilized - 100 µg - $145.00
aa304-318
Human GNAT3.
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 27kD band in Human Colon and Testis lysates and in cell lines NIH 3T3, A431 and Kelly after 0.1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 40.4kD according to NP_001095856.1. The 27kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

Publications (1)

1
Non-specific immunostaining by a rabbit antibody against gustducin alpha subunit in mouse brain. Xiong G, Redding K, Chen B, Cohen AS, Cohen NA. The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society. Feb;63:79-87. [Full Text Article] [PubMed:25411190] [PMC:PMC4305516]

About GNAT3 / Gustducin

A8MTJ3 NP_001095856.1

GNAT3 Antibody, Gustducin Antibody, Gustducin alpha-3 chain Antibody, GDCA Antibody

Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction.

Immunohistochemistry

Immunohistochemistry
Anti-GNAT3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4942 concentration 3.75 ug/ml.
 

Requested From: United States
Date Requested: 12/6/2016

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