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Anti-GNAS Antibody (aa36-85) IHC-plus™ LS-B4007

Note: This antibody replaces LS-C101987

Ordering

Wt. Vol. Conc. Price
50 µg - 1 mg/ml $450
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GNAS Antibodies

Anti-GNAS Antibody (aa51-100) LS-C31914
Rabbit Polyclonal (IgG) to Human GNAS
Human, Mouse, Rat, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GNAS Antibody (aa36-85) IHC-plus™ LS-B4007
Rabbit Polyclonal (IgG) to Human GNAS
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GNAS Antibody (aa287-315) LS-C166549
Rabbit Polyclonal to Human GNAS
Human
IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG) to Human GNAS
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig
IHC - Paraffin, Western blot
Unconjugated

Details

Human GNAS
Rabbit
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Hamster, Horse, Pig (tested or 100% immunogen sequence identity)
IgG Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (5 µg/ml)
  • Western blot (1 µg/ml)

Specificity and Use

GNAS antibody was raised against synthetic peptide located between aa36-85 of human GNAS (P63092, NP_000507). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Elephant, Dog, Bovine, Bat, Horse, Pig, Guinea pig (100%).
LS-E15725 - Lyophilized - 100 µg - $145.00
aa36-85
Human GNAS
Immunohistochemistry: LS-B4007 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B4007 was determined to be 5 ug/ml. Western Blot: Suggested dilution at 1µg/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500.

Packaging

Lyophilized from PBS with 2% sucrose
Distilled water
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year); Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
For research use only.

About GNAS

Q5JWF2 NM_080425 NP_536350.2

GNAS Antibody, AHO Antibody, C20orf45 Antibody, Extra large alphas protein Antibody, GSP Antibody, GNAS complex locus Antibody, GNASXL Antibody, NESP Antibody, PHP1B Antibody, PHP1C Antibody, PHP1A Antibody, POH Antibody, SCG6 Antibody, XLalphas Antibody, GNAS1 Antibody, GPSA Antibody, GSA Antibody, NESP55 Antibody, Protein ALEX Antibody, Secretogranin VI Antibody

The GNAS1 gene encodes the alpha subunit of the G protein Gs, which couples receptor binding by several hormones to activation of adenylate cyclase (Hayward et al., 1998). This locus has a highly complex imprinted expression pattern. It gives rise to maternally, paternally, and biallelically expressed transcripts that are derived from four alternative promoters and 5' exons.

Immunohistochemistry

Immunohistochemistry
Anti-GNAS antibody IHC of human thyroid. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4007 concentration 5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-GNAS antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4007 concentration 5 ug/ml.

Western blot

Western blot
Western blot of MCF7 cell lysate.
 

Requested From: United States
Date Requested: 12/11/2016

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