Target
Human GM2A
Synonyms
GM2A | Ganglioside GM2 activator | GM2 ganglioside activator | GM2-AP | SAP-3
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TTGNYRIESVLS, from the internal region of the protein sequence according to NP_000396.2.
Specificity
Human GM2A. This antibody is expected to recognize isoform 1 (NP_000396.2) only.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:4000)
Usage
Peptide ELISA: antibody detection limit dilution 1:4000. Western blot: In transfected HEK293 transiently expressing full-length Human GM2A (myc and DYKDDDDK tagged), a band of approx. 28kD was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-DYKDDDDK tag antibody. Recommended concentration, 1-2 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee