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Anti-GLUD1/Glutamate Dehydrogenase Antibody LS-C59301

Ordering

Wt. Vol. Conc. Price
- 2000 µl 85 mg/ml $1,110
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular GLUD1/Glutamate Dehydrogenase Antibodies

Anti-GLUD1/Glutamate Dehydrogenase Antibody IHC-plus™ LS-B2525
Rabbit Polyclonal (IgG) to Bovine GLUD1/Glutamate Dehydrogenase
Bovine, Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image
Anti-GLUD1/Glutamate Dehydrogenase Antibody (aa182-231) IHC-plus™ LS-B4661
Rabbit Polyclonal (IgG) to Human GLUD1/Glutamate Dehydrogenase
Human, Mouse, Rat, Bovine, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-GLUD1/Glutamate Dehydrogenase Antibody (aa93-107) IHC-plus™ LS-B8318
Goat Polyclonal to Human GLUD1/Glutamate Dehydrogenase
Human, Monkey, Mouse, Horse, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Bovine GLUD1/Glutamate Dehydrogenase
Bovine
Western blot, ELISA
Unconjugated

Details

Bovine GLUD1/Glutamate Dehydrogenase
Rabbit
Bovine (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Delipidated and defibrinated
Unmodified

Applications

  • Western blot (1:1000 - 1:3000)
  • ELISA (1:4000 - 1:16000)

Specificity and Use

GLUD1/Glutamate Dehydrogenase antibody was raised against full length Glutamate Dehydrogenase protein isolated from Bovine Liver.
Glutamate Dehydrogenase [Bovine Liver]. BLAST analysis was used to determine that cross reactivity is suggested for both mitochondrial and brain isoforms (GDH1 and GDH2), from both bovine and human sources. Additionally similar reactivity is suggested for most primate species including green monkey, white gibbon, chimpanzee orangutan, and gorilla. A high degree of sequence homology is also noted for GDH from chicken, mouse, rat, dog, and other mammals as well as Xenopus tropicalis, zebrafish, rainbow trout and Atlantic salmon. Cross reactivity against Glutamate Dehydrogenase from other tissues and species may occur but have not been specifically determined
This antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Bovine glutamate dehydrogenase exists as a homohexamer located within the mitochondrial matrix. Expect a band approximately 56 kD in size corresponding to glutamate dehydrogenase monomer subunit by western blotting in the appropriate cell or tissue extract.

Packaging

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
Store at 4°C.
For research use only.

About GLUD1/Glutamate Dehydrogenase

P00367 NM_005271 NP_005262.1

GLUD1 Antibody, GDH1 Antibody, GDH 1 Antibody, Glutamate dehydrogenase 1 Antibody, Glutamate dehydrogenase 2 Antibody, GDH Antibody, GLUD Antibody

GLUD1/Glutamate Dehydrogenase is glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP.

Western blot

Western blot
Anti-Bovine Glutamate Dehydrogenase Antibody - Western Blot. Western blot analysis is shown using anti-bovine glutamate dehydrogenase antibody to detect the enzyme from bovine liver preparations. Comparison to a molecular weight marker indicates a predominant band of ~62 kD. The higher molecular weight band may represent a subunit dimer. A 4-20% gradient gel was used to separate proteins prior to transfer to 0.2 micron nitrocellulose. The blot was incubated with a 1:1000 dilution of the antibody for 2 h at room temperature followed by detection using IRDye800 labeled Goat-a-Rabbit IgG [H&L] ( diluted 1:5000 for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
 

Requested From: United States
Date Requested: 12/11/2016

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