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Anti-GLUD1/Glutamate Dehydrogenase Antibody (aa93-107) IHC-plus™ LS-B8318

Note: This antibody replaces LS-C112934
Catalog Size Price
LS-B8318-50 50 µg (0.5 mg/ml) Unavailable

Most Popular GLUD1/Glutamate Dehydrogenase Antibodies

Anti-GLUD1/Glutamate Dehydrogenase Antibody LS-C41934
Rabbit Polyclonal (IgG) to Bovine GLUD1/Glutamate Dehydrogenase
Bovine
Western blot, ELISA
Unconjugated
Anti-GLUD1/Glutamate Dehydrogenase Antibody IHC-plus™ LS-B2525
Rabbit Polyclonal (IgG) to Bovine GLUD1/Glutamate Dehydrogenase
Bovine, Human
IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-GLUD1 antibody IHC of human pancreas. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2525 concentration 5 ug/ml.
Anti-GLUD1/Glutamate Dehydrogenase Antibody (aa182-231) IHC-plus™ LS-B4661
Rabbit Polyclonal (IgG) to Human GLUD1/Glutamate Dehydrogenase
Human, Mouse, Rat, Bovine, Horse, Pig, Rabbit, Chicken, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Anti-GLUD1 antibody IHC of human colon, myenteric plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4661 concentration 5 ug/ml.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Anti-GLUD1/Glutamate Dehydrogenase Antibody (aa93-107) IHC-plus™ LS-B8318
Goat Polyclonal to Human GLUD1/Glutamate Dehydrogenase
Human, Monkey, Mouse, Horse, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8318 dilution 3.75 ug/ml.
Anti-GLUD1/Glutamate Dehydrogenase Antibody LS-C482853
Rabbit Polyclonal to Human GLUD1/Glutamate Dehydrogenase
Human, Mouse, Rat
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemical analysis of GLUD1 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

100% Guaranteed
Goat Polyclonal to Human GLUD1/Glutamate Dehydrogenase
Human, Monkey, Mouse, Horse, Pig
IHC - Paraffin, Western blot, ELISA
Unconjugated

Details

Human GLUD1/Glutamate Dehydrogenase
Goat
Human, Monkey, Mouse, Horse, Pig (tested or 100% immunogen sequence identity)
Rat (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (3.75 µg/ml)
  • Western blot (0.3 - 1 µg/ml)
  • ELISA (1:32000)

Specificity and Use

Synthetic peptide C-ESEEQKRNRVRGILR from an internal region of human GLUD1 (NP_005262.1; NP_036216.2). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Monkey, Marmoset, Mouse, Panda, Horse, Pig (100%); Rat, Opossum, Pufferfish (93%); Bovine, Xenopus, Stickleback (87%).
LS-E27293 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B8318. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
aa93-107
Human GLUD1. This antibody is expected to recognize both reported isoforms (NP_005262.1; NP_036216.2).
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 55kDa band observed in Human Liver and in Mouse Liver lysates (calculated MW of 61.4kDa according to Human NP_005262.1 and NP_036216.2 and of 61.3 according to Mouse NP_032159.1. Recommended concentration: 0.3-1 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.

About GLUD1/Glutamate Dehydrogenase

P00367 NM_005271 NP_005262.1

GLUD1 Antibody, GDH1 Antibody, GDH 1 Antibody, Glutamate dehydrogenase 1 Antibody, Glutamate dehydrogenase 2 Antibody, GDH Antibody, GLUD Antibody

GLUD1/Glutamate Dehydrogenase is glutamate dehydrogenase protein; a mitochondrial matrix enzyme that catalyzes the oxidative deamination of glutamate to alpha-ketoglutarate and ammonia. This enzyme has an important role in regulating amino acid induced insulin secretion and activating mutations in this gene are a common cause of congenital hyperinsulinism. This enzyme is allosterically activated by ADP and inhibited by GTP and ATP.

Immunohistochemistry

Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8318 dilution 3.75 ug/ml.
Anti-GLUD1 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B8318 dilution 3.75 ug/ml.

Western blot

EB09856 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09856 (0.01µg/ml) staining of Human (A), Mouse (B) and Rat (C) Liver lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

EB09856 (0.03µg/ml) staining of Human (A) and Mouse (B) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09856 (0.03µg/ml) staining of Human (A) and Mouse (B) Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot

GLUD1 antibody (0.3 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
GLUD1 antibody (0.3 ug/ml) staining of Mouse Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Requested From: 
Date Requested: 4/19/2018

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